For outstanding optical and thermal performance for high-quality results on the Rotor-Gene Q
The Q-Rex Gene Expression Plug-in allows you to perform relative quantitation analyses, based on the mathematical model suggested by Pfaffl. This model is a more generalized application of the widely-used comparative C
T method (2
-ΔΔCT) described by Livak and Schmittgen*.
- Normalization to more than one reference gene
- Adjustment of the real-time PCR reaction efficiency
The amount of a target gene is set in relation to a defined calibrator sample and subsequently normalized to one or multiple reference genes. The calibrator sample could be, for example, wild-type, untreated control or time-zero samples.
View your reference genes in the plot for evaluation.
For use with the following kits:
- QuantiNova SYBR® Green PCR and RT-PCR kits
- QuantiFast SYBR Green PCR and RT-PCR Multiplex kits
- RT2 qPCR assays and arrays
- miScript and miRCURY assays and arrays
* Livak, K.J. and Schmittgen, T.D. (2001) Analysis of relative gene expression data using realtime quantitative PCR and the 2^[-delta delta C(T)] method. Methods 25, 402.