Digital PCR

The new absolute in PCR – Digital PCR

The power of digital PCR – absolute quantification in less than 2 hours

Digital PCR (dPCR) is a technique where the sample is partitioned into many individual reactions so that either zero, one or more target molecules are present in each reaction. This approach makes it surprisingly easy to detect a single positive against a strong background of negatives. In such instances, the lower limit of detection excludes standard real-time quantitative PCR (qPCR) as a viable method. Even more, dPCR doesn’t rely on standard curves and has a higher precision than qPCR.

Researchers increasingly use the digital PCR system for sensitive applications. The method has helped advance cell and gene therapy research, cancer research, infectious disease surveillance, food testing and many more.

It is easy to use the QIAcuity

Transition into a simple and rapid digital PCR workflow.

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Nanoplate dPCR technology

Unveiling the magic inside

Sample partitioning methods in digital PCR include microfluidic chips, microarrays, spinning microfluidic discs, and droplet techniques based on oil-water emulsions. Reimagining familiarity, ease of use and finer resolution, we have developed microfluidic nanoplates to achieve superior partitioning and enhanced digital PCR data.

No droplets. No crystals. No chips.

The key advantages of performing digital PCR in nanoplates include but are not limited to:

User-friendly, familiar plates are easy to pipet just like for qPCR
Fixed partitions prevent variation in size and coalescence
Sealed nanoplates prevent well to well contamination
Faster readout possible due to simultaneous reading of all partitions of a sample
Integrated quality control, meaning one can look at the plate image for single fluorescent signals from the individual positive partition counts
Plates are amenable to front-end automation

Advantages of the QIAcuity Digital PCR System

Testimonials – what our customers say

FAQs about QIAcuity Nanoplate Digital PCR

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