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Digital PCR

Applications

Discover - Top applications

Applications of Digital PCR

Thanks to the high sensitivity, superior precision and absolute quantification, digital PCR can detect low abundance targets, targets in complex mixtures, allelic variants and monitor small fold-change differences in target levels across a wide range of samples and applications, including but not limited to:

  • Copy number variation
  • Rare mutation detection
  • Gene expression and miRNA analysis
  • Microbial pathogen detection
  • NGS validation
  • GMO detection

Digital PCR allows for increased sensitivity in the detection and absolute quantification of rare events/sequence variants because target quantification is independent of the number of amplification cycles. Besides, partitioning increases the signal-to-noise ratio and decreases false-positive rates when detecting low-frequency targets (allelic variants, SNPs) in a pool of wild-type background.

Emerging uses of digital PCR for rare mutation detection include:

  • Identifying residual cancer below current detection levels
  • Detecting new mutations and duplications in cancer
  • Monitoring rare drug-resistance mutations

Digital PCR can easily detect small fold-change differences such as less than a 1.2-fold change in CNV from four to five copies, without requiring a standard curve. The precision and sensitivity of the technology allows the system to distinguish subtle changes in copy number, and the accuracy is less sensitive to changes in amplification efficiency and presence of inhibitors. Emerging uses of digital PCR for copy number variation lie in the field of cancer research (screening and monitoring of cancer biomarkers), research into neurological conditions, and population studies.

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Digital PCR tips and tricks

Find a compilation of the top 20 questions, along with insightful answers from our guest webinar.

The increased precision of digital PCR can provide higher resolution in many aspects of gene expression analysis. It enables monitoring finer changes in expression levels, even less than two-fold. Digital PCR also provides a greater sensitivity when quantifying rare targets, or RNA from very limited starting material.

Emerging uses of digital PCR for gene expression analysis include:

  • Studying methylation events
  • Quantifying transcription levels
  • Measuring changes in miRNA levels
gene expression analysis
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