Product Groups – Discover featured QIAcuity products
Guide to digital PCR multiplexing
All you need to know about dPCR multiplexing, from benefits and applications to tips and products for adopting this approach in your lab.
QIAcuity instruments – Features and specifications
| QIAcuity One | QIAcuity Four | QIAcuity Eight | |
|---|---|---|---|
| Plates processed | 1 | 4 | 8 |
| Detection channels (multiplexing) | 2 or 5 | 5 | 5 |
| Thermocycler(s) | 1 | 1 | 2 |
| Time to result | Approx. 2h | First plate approx. 2 h Every ~80 min a following plate |
First plate approx. 2 h Every ~40 min a following plate |
| Throughput (samples processed in a work day) | Up to 384 (96-well) Up to 96 (24-well) |
Up to 672 (96-well) Up to 168 (24-well) |
Up to 1248 (96-well) Up to 312 (24-well) |
Unveiling the magic inside
Reimagining familiarity, ease of use and finer resolution, we have developed these nanoplates with superior partitioning, meaning unparalleled precision and sensitivity gain for your assays.
Nanoplates – Features and specifications
| Specifications | Applications | |
|---|---|---|
| Nanoplate 26K 8-well | 8-well x approx. 26,000 partitions | Rare mutation detection, liquid biopsy, pathogen detection, etc. |
| Nanoplate 26K 24-well | 24-well × approx. 26,000 partitions |
Rare mutation detection, liquid biopsy, pathogen detection, etc. |
| Nanoplate 8.5K 24-well | 24-well × approx. 8500 partitions |
CNV determination, NGS library quantification, genome edit detection, etc. |
| Nanoplate 8.5K 96-well | 96-well × approx. 8500 partitions |
CNV determination, NGS library quantification, genome edit detection, etc. |
Kits and reagents – Features and specifications
| Specifications | Applications | |
|---|---|---|
| QIAcuity Probe PCR Kit | For single and multiplex use (1- to 5-plex) | Optimized for best performance in nanoplate microfluidic Includes special reference dye needed for dPCR analysis and counting analyzable partitions |
| QIAcuity EG PCR Kit | EG-based master mix | Optimized for best performance in nanoplate microfluidic Includes special reference dye needed for dPCR analysis and counting analyzable partitions |
| QIAcuity UCP Probe PCR Kit | Nucleic acid-depleted, ultra-clean reagents | Optimized for processes that are prone to contaminating background DNA Includes ready-to-use Master Mix for microfluidic use in singleplex or up to 5-plex analysis |
| QIAcuity OneStep Avanced Probe Kit | For one-step RT-PCR | Optimized for quantification of RNA or RNA+DNA targets Includes HotStart Reverse Transcription (RT) Enzyme for room-temperature reaction setup and simultaneous Nanoplate setup |
*For RNA samples and cDNA synthesis, the QuantiTect Reverse Transcription Kit is recommended.
Not sure which digital PCR products to pick for your application?
This smart online tool can help you find the right instrument and consumables to match your workflow needs.
Publications
Browse a growing list of articles featuring QIAcuity dPCR products.
- Huggett JF et al. (2022) Monkeypox: another test for PCR. Euro Surveill., 27(32).
- Amman, F et al. (2022) Viral variant-resolved wastewater surveillance of SARS-CoV-2 at national scale. Nat Biotechnol.
- Tasnim T et al. (2022) A duplex PCR assay for authentication of Ocimum basilicum L. and Ocimum tenuiflorum L in Tulsi churna. Food Control. Volume 137, 108790.
- Alexandra S et al. (2022) Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics. Methods. Volume 201, Pages 5-14.
- Nyaruaba R et al. (2022) Digital PCR applications in the SARS-CoV-2/COVID-19 era: a roadmap for future outbreaks. Clin Microbiol Rev. Sep 21;35(3):e0016821.
- Sun N et al. (2022) Coupling lipid labeling and click chemistry enables isolation of extracellular vesicles for noninvasive detection of oncogenic gene alterations. Adv. Sci., 9, 2105853.
- Piao XM et al. (2022) Expression of RPL9 predicts the recurrence of non-muscle invasive bladder cancer with BCG therapy. Urol Oncol. May;40(5):197.e1-197.e9.
- Zaytseva M et al. (2022) Methodological challenges of digital PCR detection of the histone H3 K27M somatic variant in cerebrospinal fluid. Pathol Oncol Res. Apr 12;28:1610024.
- Christoph S et al. (2022) Effects of varying flux and transmembrane pressure conditions during ceramic ultrafiltration on the infectivity and retention of MS2 bacteriophages. Separation and Purification Technology. Volume 299, 121709.
- Lindsey AM et al. (2022) Digital polymerase chain reaction strategies for accurate and precise detection of vector copy number in chimeric antigen receptor T-cell products. Cytotherapy.
- Boogaerts T et al. (2021). An alternative approach for bioanalytical assay optimization for wastewater-based epidemiology of SARS-CoV-2. Science of The Total Environment,Volume 789, Article 148043.
- Luo Y et al. (2020). Massively parallel single-molecule telomere length measurement with digital real-time PCR. Sci Adv. 6(34):eabb7944.
- Lee EG et al. (2021). TOMM40 RNA Transcription in Alzheimer’s Disease Brain and Its Implication in Mitochondrial Dysfunction. Genes. 12, 871.
- Wirtz RM et al. (2021). FGFR testing from matched tissue and urine samples within the prospective real world clinico-pathological register trial BRIDGister. Journal of Clinical Oncology. 39:15_suppl, e16532-e16532.
- [Preprint version only] Yang JX et al. (2021). Digital PCR quantification of DNA, RNA and extracellular microRNA of mouse oocytes.
- Ferasin L et al. (2021). Infection with SARS-CoV-2 variant B.1.1.7 detected in a group of dogs and cats with suspected myocarditis. Vet Rec. 2021 Nov;189(9):e944.
- [Preprint version only] Wu X et al. (2021). A Warm-start Digital CRISPR-based Method for the Quantitative Detection of Nucleic Acids.
- [Preprint version only] Viveros ML et al. (2021). Wild Type and Variants of Sars-Cov-2 in Parisian Sewage: Dynamics in Raw Water and Fate in Wastewater Treatment Plants.
- Romanelli K et al. (2021) Clinical and molecular characterization of thyroid cancer when seen as a second malignant neoplasm. Therapeutic Advances in Endocrinology and Metabolism. Volume: 12
- Murphy LA et al. (2021) Detection of Vector Copy Number in Bicistronic CD19xCD22 CAR T Cell Products with Digital PCR. Blood. 138 (Supplement 1): 4001.
- [Preprint version only] Toffoli M et al. (2021) Comprehensive analysis of GBA using a novel algorithm for Illumina whole-genome sequence data or targeted Nanopore sequencing.
- Passera A et al. (2021) Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot. Microorganisms, 9(11), 2388.
How to choose a QIAcuity Digital PCR System
Together with its nanoplates, kits and assays, each instrument delivers high performance and data quality, with flexible throughput and multiplexing. Ask the right questions to evaluate your needs.
- What applications will you perform most frequently?
- Which instrumentation options best enable your scale of study?
- What are the costs for product care beyond the initial investment?
For further guidance about comprehensive product care when making your decision, download the QIAcuity Service Brochure.
Can your research benefit from having QIAcuity?
Make the right choice for your laboratory.
*The GT-Digital SARS-CoV-2 Wastewater Surveillance Assay For QIAcuity is currently available in the United States and Canada. Contact your country representative for product availability details. GT Molecular is The Molecular App Company™.
The QIAcuity is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood banking) is unknown.