PCR Enzymes & Kits
Highly specific and sensitive PCR
When performing PCR, your experiments’ success can be impacted by a number of critical factors including the throughput, assay sensitivity, robustness of the reaction even with difficult templates, and reliable data normalization. Difficult templates with a high GC content, low template amounts and the need to optimize reaction conditions pose additional challenges.
Our PCR reagents and PCR enzymes guide your success and help you overcome the challenges you experience. The dual-cation PCR buffer in our PCR kits maintains a high primer annealing specificity over a broad range of temperatures which means you don’t have to optimize the PCR for each individual primer–template system.
Product category | Your application goal | Recommended products | When to choose |
|---|---|---|---|
| Hot start PCR |
| For robust, reliable amplification of standard targets | |
| Multiplex PCR | Amplify multiple DNA targets in one reaction | When detecting several targets simultaneously and reducing sample input | |
| One-step RT-PCR | Translate RNA target into DNA in one reaction (RT + PCR) | When starting from RNA and no separate cDNA storage is required | |
| Reverse transcription | Convert RNA into cDNA for downstream applications | When flexible downstream assays or long-term storage of cDNA is needed | |
| High fidelity & long range PCR | High-fidelity amplification for sequencing or cloning | When sequence accuracy or long amplicons (>5–10 kb) are required | |
| PCR enzymes (bulk & OEM) | Custom assay development or OEM supply | For specific or industrial applications and custom formulations |
Find the right end-point PCR product for your workflow
Once you’ve identified the right solution for your application, use the table below to explore detailed specifications of individual PCR kits and enzymes.
It provides key technical parameters such as template type, amplicon length, fidelity, multiplex capability, and performance characteristics — helping you select the most suitable product for your specific workflow.
Product | Template | Amplicon length | Exonuclease activity | Resulting ends | Proof-reading | Multiplex capability | Hot start | Extension time | NGS-ready | GC-Rich or difficult templates |
|---|---|---|---|---|---|---|---|---|---|---|
| Hot Start PCR HotStarTaq DNA Polymerase | DNA | 3–4 kb | Yes 5'→3' | 3' dA overhang | No | No | Yes Chemically-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Hot Start PCR AllTaq PCR Core Kit | DNA | Up to 9 kb | Yes 5'→3' | 3' dA overhang | No | Yes 2 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Multiplex PCR QIAGEN Multiplex PCR Kit | DNA | ∼2–3 kb | Yes 5'→3' | 3' dA overhang | No | Yes Up to 16 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Multiplex PCR UCP Multiplex PCR Kit | DNA | ∼3–4 kb | Yes 5'→3' | 3' dA overhang | No | Yes Up to 22 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| One-step RT-PCR QIAGEN OneStep RT-PCR Kit | RNA | ≤ 2 kb | Yes 5'→3' | 3' dA overhang | No | No | Yes Chemically-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| One-step RT-PCR QIAGEN OneStep Ahead RT-PCR | RNA | ≤ 4 kb | Yes 5'→3' & 3'→5' | Mixture, 3′ dA overhang and blunt ends | Yes | Yes 2 targets | Yes Antibody-mediated | Fast cycling (∼60 min protocol) | No | Yes With Q-Solution |
| Reverse transcription Omniscript RT Kit | RNA | 1–3 kb | N/A | N/A | N/A | No | No | N/A | No | Yes |
| Reverse transcription EnzScript Reverse Transcriptase | RNA | cDNA transcripts >9.4 kb | N/A | N/A | N/A | No | No T° tolerance up to 50°C | N/A | Yes library construction for RNA-Seq transcriptome studies | No |
| High fidelity & long range PCR UltraRun LongRange PCR Kit | DNA | Up to 30 kb | Yes 3'→5' | Blunt ends | Yes [+dU tolerant] | Yes Up to 6 targets | Yes Antibody-mediated | ~1kb / 30s | No | Yes With Q-Solution |
| High fidelity & long range PCR 2x Hi-Fi PCR Master Mix | DNA | Up to 4 kb | Yes 3'→5' | Blunt ends | Yes | Yes library amplification | Yes Antibody-mediated | ~1kb / 30s | Yes NGS library amplification | Yes |
| High fidelity & long range PCR VeraSeq PCR Mix | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes | Yes Can used for higher order amplicon pools for sequencing | No | ~1kb / 15s | No | No |
| High fidelity & long range PCR VeraSeq 2.0 High-Fidelity DNA Polymerase | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes | Yes Can be used for higher order amplicon pools for sequencing | No | ~1kb / 15s | No | Yes with 5X VeraSeq GC buffer |
| High fidelity & long range PCR VeraSeq 2.0 Ultra DNA Polymerase | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes [+dU tolerant] | Yes Can be used for higher order amplicon pools for sequencing | No | ~1 kb / 15s | No | Yes with 5X VeraSeq GC buffer |
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21 Products found
VeraSeqâ„¢ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
VeraSeqâ„¢ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR
2x HiFi PCR Master Mix
A high efficiency, high-fidelity and low bias PCR master mix for NGS library amplification.
VeraSeqâ„¢ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
QIAGEN OneStep RT-PCR Kit
For highly sensitive and specific one-step RT-PCR
QIAGEN Multiplex PCR Kit
For highly specific and sensitive multiplex PCR without optimization requirements
HotStarTaq DNA Polymerase
For highly specific amplification with minimal optimization
HotStarTaq Master Mix Kit
For highly specific amplification for any PCR application
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
Taq DNA Polymerase
Taq DNA Polymerase delivers fast PCR set up with minimal optimization for efficient amplification of routine, low-copy, longer or difficult targets
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
Omniscript RT Kit
For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
Sensiscript RT Kit
For reverse transcription of less than 50 ng RNA per reaction for end-point PCR
HotStarTaq Plus DNA Polymerase
For fast and highly specific amplification in all applications
Taq PCR Master Mix Kit
Taq PCR Master Mix Kit is a 2x solution of Taq DNA polymerase, dNTPs, MgCl2 and PCR buffer to simplify your workflow, reduce contamination risk and improve throughput and reproducibility
HotStarTaq Plus Master Mix Kit
For fast and highly specific amplification in all applications
UltraRun LongRange PCR Kit
For ultrafast, hot-start mediated, long-range PCR
QIAGEN OneStep Ahead RT-PCR Kit
For faster one-step RT-PCR with high sensitivity, specificity and fidelity
UCP Multiplex PCR Kit
Ultra-Clean Production master mix for multiplex hot-start PCR and microbiome applications
Type-it Mutation Detect PCR Kit
For accurate and reliable multiplex PCR analysis of mutations
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