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Transfection Cell Database - Detailed View


Cell Line: 293

Cell Line Species/Tissue: human / kidney epithelial 
Transfection Reagent: PolyFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM containing Glutamax, 4,5g Glucose, 10%FCS, 1%NEAA 
Percent Serum (%):
10 
Reporter System:
b-gal 
Plasmid Purification Method:
 
Plate Format:
75cm3 
Number of Cells:
 
Percent Confluence(%):
60-80 
Amount of Nucleic Acid (µg):
12 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
115 
Complex Incubation on Cells (hrs):
24/48 
Analysis Performed Post-Transfection (hrs):
24/48 
Transfection Efficiency (%):
90% 
 
Any modifications to the protocol?:
Wash cells once with 15ml PBS during complex formation Transfection in a T75 flask  
Notes:
 
References: