Products
Experiment Configurator
Discovery & Translational Research
DNA & RNA Purification
DNA
Cell-Free DNA
DNA Clean Up
Genomic DNA
Microbial DNA
Plasmid DNA
RNA
Cell-Free RNA
Microbial RNA
miRNA
mRNA
RNA Clean Up
Total RNA
Multianalyte & Virus
Instruments & Equipment
Protein Purification
Fractionation & Depletion
Tagged Protein Expression, Purification, Detection
Exosomes & CTCs
Circulating Tumor Cells
Exosomes
Enzymes for Molecular Biology
Enzymes PCR and DNA amplification
Enzymes RNA analysis
Enzymes assay and detection
Enzymes reaction cleanup and yield improvement
Enzymes cloning and recombinant DNA technology
Enzymes nucleic acid labeling
Sample Collection & Stabilization
DNA
RNA
Tissue/FFPE
QIAcard FTA
PCR/qPCR/dPCR
PCR Enzymes & Kits
End-Point PCR
HiFidelity, Long-Range & Other PCR
One-Step RT-PCR
Reverse Transcription & cDNA Synthesis
Real-Time PCR Enzymes & Kits
Probe-Based One-Step qRT-PCR
Probe-Based qPCR
Reverse Transcription & cDNA Synthesis for qPCR
SYBR Green- or Dye-Based One-Step qRT-PCR
SYBR Green- or Dye-Based qPCR
qPCR Assays & Instruments
CNV qPCR Assays & Arrays
Microbial DNA qPCR Assays & Panels
miRNA qPCR Assay & Panels
mRNA/IncRNA qPCR Assays & Panels
Somatic Mutations qPCR Assays & Panels
Food Safety Testing
Preamplification
Preamplification Kits
REPLI-g
dPCR Assays Kits & Instruments
dPCR Instruments
dPCR Kits
dPCR Assays
dPCR Accessories
Custom LNA Oligonucleotides
Next-Generation Sequencing
DNA Sequencing
Whole Genome Sequencing
Whole Exome Sequencing
Targeted DNA Panels
Single Cell DNA
Custom DNA Panels
Whole Genome
RNA Sequencing
3’ RNAseq
miRNA & Small RNAseq
RNA Fusions
Stranded RNAseq
Targeted RNA Panels
T-Cell Receptor Sequencing
Single Cell RNA
Ultraplex 3’ Targeted
Ribosomal RNA & Globin mRNA Removal
Custom RNA Panels
Multianalyte Sequencing (Multimodal)
Multimodal Panels
Single Cell DNA/RNA
Custom Multimodal Panels
Sample Quality Control
Whole Genome/Transcriptome Amplification
Single Cell & Low Input
Whole Genome
Whole Transcriptome
DNA Methylation Analysis
Metagenomics
Whole Genome Metagenomics
Targeted Metagenomics
Metatranscriptome Analysis
Informatics & Data
Pyrosequencing
ISH & Northern Blotting
Epigenetics
Chromosome Conformation
DNA Methylation
Bisulfite Conversion Assays
Methylation-Specific PCR
Pyrosequencing
DNA Methylation Analysis
Histone Modification
Functional & Cell Analysis
ELISA Assays
Gene Reporter Assays
miRNA Functional Analysis
RNA Silencing
Transfection
CRISPR
Lab Essentials
Buffers & Reagents
Centrifuges
Enzymes
Gel Electrophoresis
Magnets
Plastics
Vacuum Manifolds & Accessories
GeneGlobe Design & Analysis Hub
Diagnostics & Clinical Research
TB Management
QuantiFERON-TB Gold Plus
Transplant
QuantiFERON Transplant
artus Viral Load
Infectious Disease
QIAstat-Dx Syndromic Testing
NeuMoDx Automated Real-Time PCR
Oncology
therascreen Solid Tumor
ipsogen Leukemia
Circulating Tumor Cells
DNA Damage Response
Sexual & Reproductive Health
Cervical Cancer Screening
Maternal/Fetal Testing
STI Testing
Sample Processing
Solutions for Laboratory-Developed Tests
Human ID & Forensics
Forensic Sample Preparation
Sample Collection
Investigator Solutions
Human DNA Quantification
STR Technology
Automation
Next-Generation Sequencing
Pyrosequencing
Validation Services
Next-Generation Sequencing
DNA Sequencing
Whole Genome Sequencing
Whole Exome Sequencing
Targeted DNA Panels
Single Cell DNA
Custom DNA Panels
Whole Genome
RNA Sequencing
3’ RNAseq
miRNA & Small RNAseq
RNA Fusions
Stranded RNAseq
Targeted RNA Panels
T-Cell Receptor Sequencing
Single Cell RNA
Ultraplex 3’ Targeted
Ribosomal RNA & Globin mRNA Removal
Custom RNA Panels
Multianalyte Sequencing (Multimodal)
Multimodal Panels
Single Cell DNA/RNA
Custom Multimodal Panels
Sample Quality Control
Whole Genome/Transcriptome Amplification
Single Cell & Low Input
Whole Genome
Whole Transcriptome
DNA Methylation Analysis
Metagenomics
Whole Genome Metagenomics
Targeted Metagenomics
Metatranscriptome Analysis
Informatics & Data
Instruments & Automation
Nucleic Acid Purification
PCR Instruments
PCR Setup & Liquid Handling
Pyrosequencing
Quality Control & Fragment Analysis
Service Solutions
Accessories
Analytics Software
Automatable Kits
QIAcube Connect Kits
QIAcube HT Kits
QIAsymphony Kits
EZ1 Kits
EZ2 Kits
Tissue Disruption
Informatics & Data
Analysis & Visualization
Interpretation & Content Databases
Enterprise Solutions
Clinical
GeneGlobe Design & Analysis Hub
Services
Genomic Services
Service Solutions
Bioinformatics Services
Strategic partnerships
OEM Enzymes
Proteinase K
Reverse Transcriptase
DNA Polymerase
RNA Polymerase
DNA Ligases
RNA Ligases
NGS Components
PCR Mixes
Modifying Enzymes
Nucleotides
Binding Proteins
OEM PCR Master Mixes
OEM Oligonucleotides
OEM Nucleic Acid Purification
OEM Spin Columns
Top Sellers
New Solutions
Shop
Applications & Insights
Applications & Insights Overview
Research & Technology
Molecular Biology Research
Automated Sample Preparation
PCR
Digital PCR
CRISPR
Next-Generation Sequencing
Liquid Biopsy
Cancer Research
Microbiome
Core Labs
Applied & Industrial
Enzymes for Molecular Biology
Pharma & Biotech
Core Labs
Genomic Services
HID & Forensics
Digital Tools & Platforms
GeneGlobe Design & Analysis
Informatics and Data
QIAsphere Digital Connectivity
Diagnostic & Clinical
Clinical dPCR
Automated PCR
TB Management
Syndromic Testing
Infectious Disease
Oncology
Laboratory Developed Tests
Partnering for Precision Diagnostics
Gynecological & Maternal Health
Plan your experiment
Strategic partnerships
Knowledge & Support
Knowledge & Support Overview
Service Solutions
Service Agreements
QIAlab Consulting
One-Time Services
Professional Services
Events & Webinars
On-Demand Sessions
Upcoming Events & Webinars
Safety Data Sheets
Knowledge Hub
Blogs
Digital Insights
Science Matters
Bench Guide
Cell-free DNA Guide
DNA
RNA
Animal Cell Culture
Epigenetics
Next-Generation Sequencing
PCR
Plasmid
Protein
Transfection
Whole Genome Amplification
Technology & Research
LNA Technology
HRM Technology
Plasmid Resource Center
Pyrosequencing Resource Center
Sample Quality Control
Whole Genome Amplification
Oncology Companion Diagnostics
Explainer Videos & Demos
Events & Webinars
Upcoming Events & Webinars
On-Demand Sessions
Digital PCR Festival 2023
Digital PCR 23
Virtual Seminars On-request
QFT-Plus Webinar Hub
QIAseq Webinar Hub
Medical Education
Pathway Research
Quality, Environmental, Health & Safety
Certificates of Analysis
Quality Assurance and Environmental, Health & Safety
GHS Hazard Labeling
QIAGEN Exclusion Database Service
Product & Technical Support
Troubleshooting guide
Quality, Environmental, Health & Safety
Life Science Grants
New Lab Program
IVDR Support
Green Packaging
Workflow Configurator
QIAsphere Digital Connectivity
Ordering Support
Marketplace Solutions
QIAstock
My QIAGEN
Local Ordering
Terms & Conditions
Resources
Product FAQ
About QIAGEN
About Us
Our Vision
Our Customers
Our Products
Our Global Presence
Our History
Our Views
Our Leadership
Executive Committee
Supervisory Board
Scientific Advisory Board
Careers
Trainee Programs
Student Programs
Why QIAGEN
Meet the QIAGENers
Your Path to QIAGEN
Contact Us
Find jobs
Investor Relations
At a Glance
Strategy
Key Figures
Equity Story
Insights Magazine
Stock Information
Share Data
Share Price
Shareholder Structure
Analyst Coverage
Analyst Consensus
Share Buyback
FAQs
Financial Reports & SEC Filings
Financial Reports
Insights Magazine
Other Reports
Ad-Hoc Announcements
Directors Dealings
Annual General Meeting
Corporate Governance
Governance Documents
Leadership
German Corporate Governance Code
Compliance & Sustainability
Compliance
Sustainability
Events & Presentations
IR Contacts
Stock Transfer Agent
Email Alerts
Information Request Form
IR Contacts
RSS
Newsroom
Press Releases
Resources
Media kit
Video Footage
Photo Archive
Press Contact
Customer stories
Sustainability
Environment
Employees
Society
A letter from the CEO
ESG Ratings
Compliance
Report a Concern
Human Rights
Supply Chain
Contact Us
Order Support
Technical Questions
Human Resources
Business Development
Pharmaceutical Industry
Media Inquiries
Investor Relations Contacts
Global Contacts
QIAGEN Subsidiaries
Commercial Partner and Distributor Solutions
QIAGEN Divisions
My QIAGEN
Cart
Technical Information Seven challenges to successful one-step RT-PCR
印刷
Bookmark
シェア
more..
pdf
882KB
English
フォーマット
ファイルサイズ
言語
ダウンロード
Get Adobe Reader
Other available languages
Contact QIAGEN
Global contacts
Technical Service
Customer Care
Related products
QIAGEN OneStep
Ahead
RT-PCR Kit
ShowResource