QIAgility

For rapid, high-precision automated PCR setup

S_1175_IAS_Qgility1659
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QIAgility HEPA/UV (w/o PC) System

Cat. No. / ID:  9001908

Robotic workstation for automated PCR setup (with UV light and HEPA filter), comes with QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts. Does not include a laptop.
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Contains
Without PC
With PC
Service Options
System
Priority
PrioPLUS
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The QIAgility, if used in combination with QIAGEN kits indicated for use with the QIAgility instrument, is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAgility instrument is used with kits other than QIAGEN kits, it is the user’s responsibility to validate the performance of such product combination for any particular application.
The QIAgility is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Features

  • Automated PCR setup in all formats
  • Convenient, easy-to-use software
  • Pre-optimized experiment setup
  • Standardized results and increased productivity
  • Elimination of manual pipetting steps

Product Details

The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use software for ease and convenience. The software provides step-by-step guidance for worktable setup and automatic calculation of all mixes, eliminating the need to program pipetting steps. Just select the kit and cycler, define your targets and start the run. For added flexibility the QIAgility is operated via a laptop computer.

Explore the virtual demo to learn more about the QIAgility.

​QIAgility operates in conjunction with QIAgility Accessories and QIAgility Consumables.

Performance

The QIAgility fully automates proven QIAGEN PCR kits for use with various workflows, and offers pure pipetting precision, enhanced safety features, and exceptional reliability and reproducibility.

Unmatched pipetting precision with QIAgility

The QIAgility instrument operated by the QIAgility Setup Manager Software demonstrates exceedingly high accuracy and precision of dispensed volumes as verified by the Artel MVS system (see figure “ Exceptional pipetting precision and accuracy spanning over a broad volume range”). These results indicate that the QIAgility can produce automated, high-performance liquid dispensing results, while avoiding common errors that can be associated with manual pipetting and thereby reducing the time to result.

Regardless of whether you pipet water or QIAGEN ready-made reaction mix to set up a PCR experiment, the QIAgility instrument can dispense volumes that are highly precise and also generate significantly reproducible replicates (see figure “ Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions”).

High performance PCR setup using QIAgility

A QuantiNova SYBR Green PCR assay setup was performed manually and automated in parallel using the QIAgility along with its Setup Manager Software. The amplification run was done on the Rotor-Gene Q and resulting data analyzed using the Q-Rex Software. Consistent results were obtained from both manual and automated reaction setup using the same template dilutions (see figure “ Comparison of manual and automated assay setup using series dilutions”).

Exceptional linearity of QIAgility

QIAgility exhibits excellent linearity and assures accurate and reliable use of sample for an assay. To assess the linearity of the system, pure gDNA was serially diluted using the QIAgility and measured for their concentrations on the QIAxpert. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity (with a regression coefficient of >0.999) of QIAgility (see figure “ High system linearity of QIAgility”).

See figures

Principle

Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that are typically prone to human error.

Liquid-handling tasks are performed by the single-channel pipetting head, which provides fast transfer of liquids with various types of plasticware. For example, 96 samples with the QuantiFast SYBR Green PCR Kit with 2 standard series can be setup in approximately 45 minutes. The worktable features a flexible layout and supports almost all kinds of cyclers and labware, giving high application versatility. Dynamic liquid-level sensing, facilitated by conductive filter tips offers maximum safety control.

The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. A built-in sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. The UV light ensures effective worktable decontamination and the HEPA filter provides positive clean air pressure throughout PCR setup.

QIAgility is a compact benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. For added flexibility, the QIAgility comes with a laptop computer. To reduce your work load, comprehensive run reports containing all run information are generated automatically during each protocol.

Procedure

Quick and easy setup of your PCR experiment with the new Setup Manager Software

​Let the QIAgility do the pipetting and save your valuable time

Applications

The instrument is highly suited for laboratories performing PCR and RT-PCR reactions with applications in: ​

  • Gene expression analysis
  • Genotyping
  • Cloning
  • Pathogen detection
  • Food and veterinary testing

The new QIAgility Setup Manager Software offers a complete solution for qPCR setup, sample normalization, CE loading, certain liquid handling, as well as sample pooling. The software includes Gene Expression, Pathogen Detection, Sample Pooling and Generic PCR plug-ins. The Gene Expression and Pathogen Detection plug-ins are optimized for use with QIAGEN gene expression and pathogen detection kits, such as QuantiNova, miRCURY and RT² kits. The Generic PCR plug-in allows flexible qPCR setup, liquid handling and support for the whole HID workflow.

Check the instrument operating software and protocol files under product resources for more information.

Services

Is your plate or tube not supported in the QIAgility software? Contact QIAGEN Technical Services to request a new plate or tube to suit your workflow.

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

Supporting data and figures

Specifications

FeaturesSpecifications
Accessories (supplied)2 x Adapter, 96 x 0.2 ml PCR Tubes 1 x Adapter, Flip-Cap 32 x 1.5 ml Tapered Base 1 x Adapter, 72 x 0.1 ml Strip Tubes 1 x Master Mix Block, 5 x 5 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 2 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 1.5 ml 1 x Reagent Block, 16 x 0.2 ml / 2 ml Flat / 1.5 ml Tapered Tubes 4 x Tip Rack Holder for 96-Tip Rack The adapters are intended to hold tubes or plates.
Conductive Filtered Tips50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing
DimensionsHeight: 45 cm (18 in.), instrument lid closed; Height: 86 cm (34 in.), instrument lid open; Width: 54 cm (21 in.); Depth: 63 cm (25 in.), without cables
SoftwareSupplied with unlimited user license. Free upgrades by Web download.
Pollution level2
TemperatureOperating temperature, 15–30 °C (59–86°F)
Pipetting headSingle channel
Tip volume1–200 µl
HEPA filterPreinstalled dual-cartridge HEPA filter system provides positive pressure clean air within the closed hood.
Liquid-level sensingMinimum detection volume (10 µl in 200 µl PCR tubes). Only functional in combination with QIAGEN Conductive Filtered Tips.
CommunicationsUSB and RS232
Consumables (supplied)10 racks of 50 µl Conductive Filtered Tips (960); 10 racks of 200 µl Conductive Filtered Tips (960); 25 x 5 ml tubes; 100 x 0.2 µl PCR tubes; 3 Tip Disposal Boxes
Electrical100–240 V AC, 250 VA, 50–60 Hz
Table capacity6 x SBS positions (127.76 x 85.48 mm) for adapters and Tip Rack Holders 1 x Master Mix Block 1 x Reagent Block
PrecisionCV <1%, 5–200 µl (dry-well transfer) CV <5%, 2–5 µl (wet-well transfer) CV <10%, 1 µl
Tip disposalAll used tips ejected externally — no waste accumulation on the worktable
Place of operationFor indoor use only
UV lampPreinstalled UV lamp for worktable decontamination
HumidityOperating humidity: 40–70%
Notebook (supplied)QIAgility comes with a notebook computer for added flexibility.
Warranty1-3 years warranty, depending on service package
Weight41 kg (90.2 lb)

Service Plans

QIAgility, Premium Agreement

Cat. No. / ID:  9241764

On-site QIAgility instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.
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QIAgility, Full Agreement

Cat. No. / ID:  9241763

On-site QIAgility instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one  Preventive Maintenance or Inspection Service during the Full Agreement period.
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QIAgility, Core Agreement

Cat. No. / ID:  9245243

For the QIAgility, one on-site repair service and one on-site Preventive Maintenance or Inspection Service, including travel, labor and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during the Core Agreement period.
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QIAgility, Preventive Subscription

Cat. No. / ID:  9241776A

One on-site Preventive Maintenance or Inspection Service visit for the QIAgility, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.
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Resources

Instrument User Manuals (5)
Important note regarding new QIAgility declaration of conformity and instrument symbols
Alternative options for the CD drive on the QIAgility Notebook, Part Number 9026761
For use with QIAgility Operating Software version 4.18
For use with QIAgility and QIAgility Setup Manager Software
Brochures & Guides (4)
Rapid, high-precision PCR setup
Addressing critical factors and new solutions
Explore our new, easy-to-navigate digital Product Profile!
Application Notes (2)
This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.
The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.
Operating Software (3)
Instrument operating software including the QIAgility Setup Manager Gene Expression, Pathogen Detection, Sample Pooling and Generic PCR plug-in. The software offers a complete solution for qPCR setup, sample normalization, CE loading, certain liquid handling, as well as sample pooling. The Gene Expression and Pathogen Detection plug-ins are optimized for use with QIAGEN gene expression and pathogen detection kits, such as QuantiTect, QuantiFast, QuantiNova and Rotor-Gene kits. The Generic PCR plug-in allows flexible qPCR setup, liquid handling and support for the whole HID workflow.

For software installation instructions, please refer to the QIAgility Unpacking and Installation Instructions or to the QIAgility User Manual, chapter 4.4.

Important
: It is not possible to install both QIAgility Setup Manager Software and QIAgility Operating Software 4.18 on the same computer. Please uninstall QIAgility Operating Software 4.18 before installing QIAgility Setup Manager Software. For more information, contact QIAGEN Technical Services.

Instrument operating software designed for flexible use in various applications. Use this software for all applications other than gene expression and pathogen detection. For gene expression and pathogen detection applications, we recommend using the QIAgility Setup Manager Software.

Important: It is not possible to install both QIAgility Operating Software 4.18 and QIAgility Setup Manager Software on the same computer. Please uninstall QIAgility Setup Manager before installing QIAgility Operating Software 4.18. For more information, contact QIAGEN Technical Services.
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?

Pros: saves tips and therefore cost.

Cons: lowers accuracy and precision as liquid is built up on the inner and outer walls of the tip with each use.

 

FAQ ID -3064
How can I decontaminate my QIAsymphony SP / AS system?

The QIAsymphony system has an integrated UV lamp. UV decontamination should be performed daily. It helps to reduce possible pathogen contamination of the QIAsymphony SP/AS worktables. The efficiency of inactivation has to be determined for each specific organism and depends, for example, on layer thickness and sample type. QIAGEN cannot guarantee complete eradication of specific pathogens.

FAQ ID - 3714
How do I transfer the QIAgility calibration settings to a different computer?

The QIAgility calibration settings can be saved using a “Setup Disk” and transferred to a different computer. This is useful and time-saving when using a different computer to run the instrument. Calibration on the new computer is not required if the calibration settings are transferred.

 

The instructions for creating and loading calibration settings from a “Setup Disk” are outlined in the QIAgility User Manual section 5.5.1.

 

Note: A “Setup Disk” created for one instrument should never be used with another instrument, as this may result in damage to the instrument.

FAQ ID -9090
What do I do if the pipettor cannot pick up tips?

Ensure the tip rack is correctly oriented and pushed towards the upper-left corner of the deck slot. Perform the tip rack position calibration.

 

If the issue persists, please contact QIAGEN Technical Services.

 

FAQ ID -9086
What do I do when QIAgility makes grinding noise?

The home position needs adjustment. Please contact the QIAGEN Technical Services for further assistance.

 

FAQ ID -9087
Can I use a bar code scanner or reader on the QIAgility?
A bar code scanner can be used when it is connected to the QIAgility computer.
FAQ ID -3067
What file is required for reporting QIAgility issue?

The Support Packages are important when reporting an issue to QIAGEN Technical Services. Upon completion of each run, the software automatically saves a support package.

 

To retrieve a support package, click Help in the QIAgility software and select Choose support package. In the new window, select the support package that corresponds to the issue and send it to QIAGEN Technical Service.

 

FAQ ID -9081
What causes Error 131?

A malfunctioning HEPA unit is the most common cause of Error 131. Shut down the system and disconnect the HEPA filter unit from the QIAgility instrument. Power on the instrument and launch the QIAgility software. If the error goes away, the HEPA filter unit needs replacing.  

 

If the issue persists, please contact QIAGEN Technical Services.

FAQ ID -9088
What do I do for error message “cannot detect liquid”?

Ensure that correct volumes of reagent are added to the tubes. Re-perform the tube height calibration.

 

If the issue persists, retrieve the support package (In the QIAgility software, click Help\Choose Support Package) and contact QIAGEN Technical Services.

FAQ ID -9083
What do I do if a red exclamation icon appears in the top-right corner of the schematic plate on the main software window?

The red exclamation icon appears if the plate has not been position- or height-calibrated, and will remain visible until all of the calibrations for that plate have been completed. Perform the position and height calibration as outlined in the QIAgility User Manual.

 

If the issue persists, please contact QIAGEN Technical Services.

FAQ ID -9089
What do I do if the pipettor hits the tip rack?

Ensure the tip rack is correctly oriented and pushed towards the upper-left corner of the deck slot. Perform the tip rack position calibration so that the pipettor hub is in the center of an empty tip position hole. Do this for all tip racks on the deck.

FAQ ID -9082
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

 

FAQ ID -2116
Can Vapor-Lock be used with samples processed on the QIAgility?

Vapor-Lock is fully compatible with the QIAgility instrument for high-precision, automated reaction setup. It is also highly suited for use with the Rotor-Gene Q cycler. For support to program your QIAgility instrument for use with Vapor-Lock, please contact your local QIAGEN Technical Support Department.

 

 

FAQ ID -2153
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?

On the QIAgility, it takes about 36 minutes if using premade master mix, and 39 minutes if the instrument needs to prepare master mix from components.

 

FAQ ID -3066
What sample tubes can I use on my QIAsymphony SP system?

Please navigate to the Product Page of the QIAsymphony Kit you are using; for example, the QIAsymphony RNA Kit, Cat No./ID: 931636.  Refer to the resources tab and download the labware document. The labware document will list the sample tubes that have been tested for your application. Please contact Technical Service if you wish to use sample tubes not listed in the labware document.

FAQ ID - 3713
Why do I do if the QIAgility software runs in virtual mode while the instrument is connected to the computer?

Make sure the QIAgility software uses the same COM port number as assigned to the QIAgility instrument on the computer. The COM port setting can be checked by clicking Options\Robot Setup\Select COM port in the QIAgility software.

 

Ensure that the cable between the QIAgility instrument and the computer is securely inserted. Power on the instrument first, and then launch the QIAgility software. 

 

The QIAgility has both RS-232 (serial) and USB interfaces for computer connection. Try an RS-232 cable if the communication cannot be established between the instrument and computer with a USB cable.

 

If the issue persists, please contact QIAGEN Technical Services.

 

FAQ ID -9041
What do I do if tips are not ejected?

Tip ejector calibration must be performed before any other calibration. Perform tip ejector calibration as outlined in the QIAgility User Manual.

 

During calibration of the tip ejector, the pipettor hub should be centered inside the slot of the tip ejector in the Y-axis — it should not touch either side.

 

If the issue persists, please contact QIAGEN Technical Services.

FAQ ID -9085