EZ1 RNA Card

预设程序卡,配合EZ1 RNA Kit在BioRobot EZ1工作站上进行RNA纯化

S_1084_5_GEN_V2

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EZ1 RNA Card

Cat. No. / ID:  9015590

Pre-programmed card for EZ1 RNA purification protocols
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EZ1 Cards配合EZ1 Kits在BioRobot EZ1工作站上使用。请参照EZ1 Kit操作手册和BioRobot EZ1用户手册,获得更多产品使用信息。
EZ1 RNA Card 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 程序设置简单,只需插卡就可以运行
  • 标准化操作流程,确保高重复性结果
  • 预设程序,提高对细胞和组织样本的处理能力
  • 经优化的操作流程,确保可靠纯化

Product Details

EZ1 RNA Card预装有纯化程序,配合EZ1 RNA Kit从细胞和容易裂解的人类或动物组织中纯化总RNA或总核酸,或从任何类型的人类或动物组织中纯化总RNA的程序。这些程序在控制面板提供操作向导,并提供BioRobot EZ1工作站的运行指令。

Performance

使用EZ1 RNA Kits和BioRobot EZ1工作站从细胞和组织中纯化获得高品质RNA和核酸适用于各种下游应用。

Principle

EZ1 RNA Card预装有纯化程序,用于从细胞和容易裂解的人类或动物组织中纯化总RNA或总核酸。EZ1 RNA Card配合EZ1 RNA Kits在BioRobot EZ1工作站上使用。

Procedure

只需在卡槽中插入预装有程序的EZ1 RNA Card并运行工作站即可。

Applications

应用EZ1 RNA Card,配合EZ1 RNA Cell Mini Kit、EZ1 RNA Tissue Mini Kit或EZ1 RNA Universal Tissue Kit获得的高品质RNA适用于各种下游应用,包括:

  • 应用real-time RT-PCR进行基因表达分析
  • 应用芯片技术进行基因表达分析
  • 肿瘤研究

Resources

快速启动实验方案 (1)
试剂盒操作手册 (1)
For purification of total RNA from tissue and cells using EZ1 instruments
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (1)
For purification of total RNA from tissue and cells using EZ1 instruments
Quick-Start Protocols (1)

FAQ

Is training of lab personnel included with the purchase of a BioRobot EZ1?

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

  • Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
  • Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
  • Collect purified nucleic acids when the protocol run ends

 

FAQ ID -1239
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?

No, the new EZ1 RNA Kits can only be used with updated protocols programmed on the new EZ1 RNA Card V2.0.

The new card contains all protocols available for the isolation of RNA on the BioRobot EZ1 with the new kits:

Likewise, the former EZ1 RNA Cell Mini Kit (cat. no. 958134), EZ1 RNA Tissue Mini Kit (cat. no. 959134) and EZ1 RNA Universal Tissue Kit (cat. no. 956136) are not compatible with the new EZ1 RNA Card (cat. no. 9015590).

FAQ ID -1025
Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
How can we get our BioRobot EZ1 upgraded when new protocols are launched?

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

 

FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

 

FAQ ID -1243
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

 

FAQ ID -1240
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

 

FAQ ID -528
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
What disposables are required for nucleic acid isolations on the BioRobot EZ1?

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

 

FAQ ID -1246