QIAcube and QIAcube Connect Accessories

For automated purification of DNA, RNA, or proteins on the QIAcube and QIAcube Connect

Features

Useful Resources

(EN) - Workflow for DNA purification from tough specimens (EN) - Automated extraction of forensic samples using established spin column technology on the QIAcube Sequential automation of RNA and DNA preps on the same QIAcube instrument

Products

Anti-corrosion oil (rotor), QIAC

Cat. No. / ID: 9018543

Anti-corrosion oil (rotor), QIAC

$75.70

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Rotor Adapter Holder

Cat. No. / ID: 990392

Holder for 12 disposable rotor adapters; for use with the QIAcube

$75.40

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Rotor Adapters (10 x 24)

Cat. No. / ID: 990394

For 240 preps: 240 Disposable Rotor Adapters and 240 Elution Tubes (1.5 ml); for use with the QIAcube

$47.00

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Filter-Tips, 1000 µl (1024)

Cat. No. / ID: 990352

Disposable Filter-Tips, racked; (8 x 128). For use with the QIAcube

$108.00

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Filter-Tips, 200 µl, (1024)

Cat. No. / ID: 990332

Disposable Filter-Tips, racked; (8 x 128). For use with the QIAcube and the QIAsymphony SP/AS instruments

$107.00

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Reagent Bottles, 30 ml (6)

Cat. No. / ID: 990393

Reagent Bottles (30 ml) with lids; pack of 6; for use with the QIAcube and QIAsymphony AS

$20.30

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Filter-Tips, 1000 µl, wide-bore (1024)

Cat. No. / ID: 990452

Disposable Filter-Tips, wide-bore, racked; (8 x 128); not required for all protocols. For use with the QIAcube

$107.00

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Elution Tubes (1.5 ml)

Cat. No. / ID: 1050875

Set of 50; for use with the QIAcube

$16.80

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Sample Tubes CB (2 ml)

Cat. No. / ID: 990382

1000 conical screw-cap tubes without skirted base (2 ml) for use with the QIAcube and QIAcube Connect

$73.90

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Sample Tubes RB (2 ml)

Cat. No. / ID: 990381

1000 safe-lock microcentrifuge tubes (2 ml) for use with the QIAcube and QIAcube Connect

$124.00

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Spin Column Adapter Rings (25)

Cat. No. / ID: 990399

Spin column adapter rings for use with spin columns that have screw caps; not required for all protocols

$154.00

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Shaker Rack Plugs (12)

Cat. No. / ID: 9017854

Shaker Rack Plugs (12)

$129.00

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Starter Pack, QIAcube

Cat. No. / ID: 990395

200 µl filter-tips (1024); 1000 µl filter-tips (1024); 30 ml reagent bottles (12); rotor adapters (240); 1.5 ml elution tubes (240); rotor adapter holder (1)

QIAcube Connect, Full Agreement

Cat. No. / ID: 9245208

On-site QIAcube Connect instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days.

Product Details

QIAcube and QIAcube Connect accessories are for use with the QIAcube and QIAcube Connect instruments for fully automated sample preparation using QIAGEN spin-column kits.

Resources

QIAcube Connect App

FAQ

For an up-to-date list of available protocols, visit QIAcube Standard Protocols.

Click here for a virtual demonstration on how the QIAcube operates.

FAQ ID -1403

Yes. The heated shaker on the QIAcube can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the shaker, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.7 'Operating the shaker'.

The shaker can be used to heat and shake samples simultaneously, shake samples without heating, or heat samples without shaking.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a virtual demonstration of the heater and shaker on the QIAcube.

FAQ ID -1422

Visit the QIAcube Protocols page to search for protocols. The instructions for transferring a protocol to the QIAcube are listed below.

To transfer new protocols to the QIAcube, first save them to the supplied USB stick.

Select the protocol to be downloaded and save it to the “New_Protocols” folder on the USB stick.
Make sure that the QIAcube is switched on.
To install the protocol, connect the USB stick to the QIAcube via the USB port, which is located at the front right of the instrument.
In the main menu, press “Tools”.
Select “Data Exchange” by pressing the "Up Symbol" or the "Down Symbol" to scroll through the list until it is highlighted and then press “Select”.
To transfer protocols to the QIAcube, select “Protocols” and “Load from USB”.
Start the download procedure by pressing “OK”. A message will confirm that the protocols have been successfully transferred to the QIAcube.

Note: Protocols already installed on the QIAcube that have the same name as downloaded protocols will be overwritten.

8. When the protocols have been successfully transferred to the QIAcube, press “OK”.

FAQ ID -1421

Yes. At the end of each protocol run on the QIAcube, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.

FAQ ID -1412

Yes. The QIAcube centrifuge can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the centrifuge, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.8 'Operating the Centrifuge'.

FAQ ID -1423

Yes, this is absolutely necessary. If the lids are not securely fixed in the rotor adapter slots during processing on the QIAcube, they will become detached from the elution tubes and columns during centrifugation and will collect at the bottom of the centrifuge chamber.

FAQ ID -1415

The following disinfectants and detergents are recommended for cleaning the QIAcube:

Mikrozid® Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g Mikrozid Liquid)
RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door
RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)
Note: Alcohol is not recommended for cleaning of the door.

Europe

Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15–30% nonionic surfactants).

USA

DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride).

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

FAQ ID -1416

New software or software updates can be accessed via the QIAcube Web Portal at www.qiagen.com/MyQIAcube. To access them directly, go to User Support.

FAQ ID -1413

No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a demonstration on how the QIAcube is used with the RNeasy Mini Kit for automated purification of RNA.

FAQ ID -1406

No. Modified QIAGEN protocols or custom protocols developed to meet your specific requirements can be requested and purchased from QIAGEN.

1. Go to www.qiagen.com/MyQIAcube and enter the required information. If our Application Specialists require additional information, they will contact you directly.

2. You will be informed by e-mail when your customized protocol is ready for download.

3. Click the link in the e-mail to go to the download page.

FAQ ID -1405

No, it is not possible to connect the QIAcube with laboratory computer networks.

FAQ ID -1411

Dedicated QIAcube kits are the RNeasy Mini QIAcube kit, QIAamp DNA Mini QIAcube Kit, the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit.

See: http://www.qiagen.com/Products/Catalog/Automated-Solutions/Sample-Prep/QIAcube#orderinginformation for a virtual demonstration on how to use the QIAcube.

FAQ ID -2337

The QIAcube has the following weight and dimensions:

Weight: 70.5 kg (155.4 lb.)
Width: 65 cm (25.6 in.)
Depth: 62 cm (24.4 in.)
Height (door closed): 57 cm (22.4 in.)
Height (door open): 81 cm (32 in.)

FAQ ID -1407

Please refer to section 6.5 'Decontaminating the QIAcube' in chapter 6 'Maintenance Procedures' of the QIAcube User Manual.

FAQ ID -1419

Yes, rotor and/or buckets can be removed from the QIAcube for cleaning. For information on how to remove these parts, refer to the QIAcube User Manual.

FAQ ID -1410

You can stop a QIAcube protocol if there is an emergency by pressing “Cancel”. To confirm that you want to stop the protocol run, press “OK”. To cancel the 'stop protocol' command, press “Cancel”.

Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.

FAQ ID -1414

The corresponding manual kits can be used for all available protocols on the QIAcube. The manual kits contain sufficient buffers and reagents to process the number of spin columns provided with the kit.

In addition to the purification kit, Rotor Adapters (10 x 24), cat. no 990394, and the required disposable tips:

Filter-Tips, 200 µl (1024), cat no. 990332,
Filter-Tips, 1000 µl (1024), cat no. 990352,
Filter-Tips, 1000 µl wide-bore (1024), cat no. 990452,

need to be ordered separately. To find out which filter-tips are required for a specific application, refer to the corresponding protocol sheet.

FAQ ID -1402

No. Autoclaving the rotor adapters will cause the plastic to deform and the rotor adapters will no longer fit properly into the centrifuge buckets.

FAQ ID -1409

No. The QIAcube is equipped with an optical sensor, which determines the tip size and the number of tips loaded onto the worktable prior to starting a run.

The QIAcube can use partially used tip racks from previous runs, provided that the racks contain sufficient tips to process the number of samples loaded for the current run.

FAQ ID -1418