QIAamp PowerFecal Pro DNA Kit
For the isolation of microbial DNA from stool and gut samples
The QIAamp PowerFecal DNA Kit removes tough inhibitors from stool, gut and biosolid samples and provides clean, ready-to-use DNA for any downstream application. With the included Inhibitor Removal Technology, inhibitors from digested food, heme from lysed red blood cells in stool and other PCR inhibitors are eliminated leaving only high-quality DNA ready for downstream applications such as qPCR, Sanger sequencing and next-generation sequencing.
The QIAamp PowerFecal DNA Kit can be used interchangeably with the DNeasy PowerSoil Kit. Both kits can be used to isolate DNA from soil, environmental, stool, gut and biosolid samples.
Isolation of DNA using the QIAamp PowerFecal DNA Kit can be automated on the QIAcube Connect. Want to try this solution for the first time? Request a quote for a trial kit. QIAamp PowerFecal DNA Kit was formerly sold by MO BIO as PowerFecal DNA Isolation Kit.
The QIAamp PowerFecal Pro DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
Using improved technologies, the new QIAamp PowerFecal Pro Kit allows researchers to obtain higher yields of DNA from stool and gut samples compared with alternative methods (See: ‘Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.’).
Improved yields of higher quality DNA are also achieved when comparing the QIAamp PowerFecal Pro DNA Kit to the first generation QIAamp PowerFecal DNA Kit when running stool and fungal samples (See: ‘The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high quality DNA.’).
Principle
The streamlined Inhibitor Removal Technology® (IRT) used by the new QIAamp PowerFecal Pro DNA Kit is highly efficient at removing inhibitors while still decreasing sample processing time. The QIAamp PowerFecal Pro DNA Kits also showed the highest A260/A280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Less variability was also observed between the samples processed with QIAamp PowerFecal Pro DNA Kits (See: ‘Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.’). Researchers using the QIAamp PowerFecal Pro DNA Kit can identify a greater bacterial diversity with increased OTUs from stool samples compared with the first-generation QIAamp PowerFecal Kit and other commercially available kits (See: ‘Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.’).
Procedure
Samples processed with the QIAamp PowerFecal Pro DNA Kit are added to a bead beating tube. Rapid and thorough homogenization occurs using mechanical and chemical methods. Once cells are lysed, our patented IRT is used to remove inhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed and eluted, ready for downstream applications.
Applications
Tools to complete your workflow:
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