FAQ-728
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.
Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).
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RNeasy Universal Kits
The RNeasy Plus Universal Mini Kit integrates fast, convenient RNA purification with effective elimination of genomic DNA. Optimized protocols enable purification of high-quality RNA from any type of tissue, even difficult-to-lyse tissues. The kit includes QIAzol Lysis Reagent for lysing fatty tissue and other types of tissue and RNeasy spin columns for purifying high-quality RNA. The RNeasy Plus Universal Mini Kit can be automated on the QIAcube Connect. The RNeasy 96 Universal Tissue Kit enables total RNA purification in 96-well format from any tissue. Tissues are efficiently lysed and homogenized in QIAzol Lysis Reagent, and high-quality RNA is purified using silica-membrane RNeasy 96 plates. Manual purification is carried out using the QIAGEN Centrifuge 4-16KS and, optionally, the QIAvac 96 vacuum manifold. Automated purification using the related RNeasy 96 Universal Tissue 8000 Kit is performed on the BioRobot Universal System. Tissue samples can be conveniently stabilized using Allprotect Tissue Reagent, and efficiently disrupted using the TissueLyser II. Nonfatty tissues can be stabilized using the RNAprotect Tissue Reagent.
RNeasy Kits
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QIAamp Viral RNA Kits
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QIAamp DNA Blood Kits
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QIAamp Circulating Nucleic Acid Kit
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RNeasy Protect Animal Blood System
The RNeasy Protect Animal Blood System provides a complete solution for preparing high-quality RNA from animal blood. Blood from mouse, rat, and other small animals is conveniently collected in RNAprotect Animal Blood Tubes, which contain a reagent that immediately stabilizes cellular RNA. Total RNA is then purified using the RNeasy Protect Animal Blood Kit. Please note that the RNeasy Protect Animal Blood Kit and the RNAprotect Animal Blood Tubes are ordered separately.Alternatively, the RNeasy Protect Animal Blood Kit can be used to obtain microRNA (miRNA), either in a total RNA fraction or in a separate small RNA fraction. For purification of total RNA containing miRNA, Buffer RWT (cat. no. 1067933) is also required. For purification of small RNA (containing miRNA) and large RNA in 2 separate fractions, the RNeasy MinElute Cleanup Kit (cat. no. 74204) is also required. The procedures can be fully automated on the QIAcube Connect.
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QIAamp Investigator BioRobot Kit
The QIAamp Investigator BioRobot Kit provides automated DNA purification from up to 96 samples (such as filters, casework or crime-scene samples, and blood) or up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot Universal System requires less than 3 hours, including bar code reading, sample tracking and complete process documentation, with no hands-on time during the run. The purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.
RNeasy Plus Kits
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QIAamp DNA Investigator Kit
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QIAamp DNA FFPE Tissue Kit
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Try the next-generation QIAamp DNA FFPE Advanced Kits for efficient recovery from DNA and optional removal of artefacts.
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In partnership with My Green Lab, we've assessed the environmental impact of the DNeasy Blood & Tissue Kit (50/250) and the QIAwave DNA Blood & Tissue Kit (50/250). My Green Lab ACT environmental impact factor labels aim to assess and rate products based on various sustainability criteria, including:
Manufacturing
Responsible chemical management
Sustainable content within products and packaging materials
Disposal of the packaging at the end of life
Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact – see figures “DNeasy Blood & Tissue Kit and QIAwave DNA Blood & Tissue Kit ACT environmental impact factor labels“ US ( 50/ 250), EU ( 50/ 250) and UK ( 50/ 250)."
RNeasy FFPE Kit
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QIAamp MinElute Media Kit
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QIAamp Media MDx Kit
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QIAamp DSP Virus Kit
The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA for in vitro diagnostic use.
RNeasy 96 BioRobot Kits
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QIAamp DSP DNA Blood Mini Kit
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The QIAGEN QIAcube Connect MDx is not available in all countries. For further details please contact QIAGEN Technical Service.
RNeasy Plant Mini Kit
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RNeasy MinElute Cleanup Kit
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RNeasy Lipid Tissue Mini Kit
The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using the QIAcube Connect. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For larger samples, the RNeasy Lipid Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.
RNeasy Fibrous Tissue Mini Kit
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QIAamp Virus BioRobot MDx Kit
The QIAamp Virus BioRobot MDx Kit provides automated viral nucleic acid purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
QIAamp Virus BioRobot 9604 Kit
The QIAamp Virus BioRobot 9604 Kit provides automated viral nucleic acid purification on the BioRobot 9604 using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours for up to 96 samples, including bar code reading and complete process documentation, with no hands-on time during the run.
QIAamp UltraSens Virus Kit
The QIAamp UltraSens Virus Kit uses a novel technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. Starting with sample volumes of up to 1 ml, nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation.
QIAamp RNA Blood Mini Kit
The QIAamp RNA Blood Mini Kit provides silica-membrane-based purification of cellular RNA from up to 1.5 ml of fresh, whole human blood stabilized with any common anticoagulant, such as citrate, heparin, or EDTA. After homogenization using the QIAshredder spin column, a fast spin-column procedure simplifies RNA purification. Purification can be fully automated on the QIAcube Connect.
QIAamp DNA Blood BioRobot MDx Kit
The QIAamp DNA Blood BioRobot MDx Kit provides automated DNA purification on the BioRobot MDx workstation or the BioRobot Universal System using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot MDx workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
QIAamp 96 DNA Swab BioRobot Kit
The QIAamp 96 DNA Swab BioRobot Kit provides automated DNA purification from up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. Up to 192 samples are processed in less than 2.5 hours. The procedure is optimized for use with air-dried swabs with plastic shafts and cotton or DACRON tips, although other swab types can be used.
DNeasy Plant Kits
The DNeasy Plant Kits provide fast and easy silica-based DNA isolation from plant samples in spin column format. Typical yields are 3–260 μg of high-quality DNA, depending on the samples used (e.g., wheat, maize, Arabidopsis, tomato, tobacco) and binding capacity of the DNeasy silica membrane. The DNeasy Plant Kits also provide silica-based DNA purification in a convenient 96-well plate format with typical yields of 1–15 μg DNA, depending on the plant species. DNA isolation from plant tissue is also automatable on the QIAcube Connect
Blood & Cell Culture DNA Kits
Blood & Cell Culture DNA Kits provide gravity-flow, anion-exchange tips and buffers for efficient isolation of genomic DNA from a wide range of biological samples. The purified DNA is sized up to 150 kb with an average size of 50–100 kb.
The purchase of the Genomic DNA Buffer Set (cat.no. 19060) is required for yeast and bacteria samples.
QIAwave RNA Mini Kit
The QIAwave RNA Mini Kit is an eco-friendlier version of our standard RNeasy Mini Kit. The kit uses up to 60% less plastic and up to 57% less cardboard than our standard kit and offers waste tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. Instead, you can download the protocols you need from the resources list or by simply scanning the QR code inside the box lid. So, while the kit packaging and components of our QIAwave Kit may look different, it’s as easy to use as the RNeasy Mini Kit, and the chemistry and performance are identical.
Please be aware that you will need sterile glass bottles to store the reconstituted buffers.
In partnership with My Green Lab, we've also assessed the environmental impact of this kit. My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. These include:
Manufacturing
Responsible chemical management
Sustainable content within products and packaging materials
Disposal of the packaging at the end of life
Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact (see figures "QIAwave RNA Mini Kit ACT environmental impact factor label US 50/250, EU 50/250 and UK 50/250").
The QIAwave RNA Mini Kit offers you fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy Mini Spin Columns. Before extraction, you can stabilize tissue samples using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and to efficiently homogenize your tissue samples, you can use the TissueRuptor II, TissueLyser III or LT system.
The QIAwave RNA Mini Kit can be automated on the QIAcube Connect using the RNeasy Mini Kit protocols.
EZ2 RNA/miRNA Tissue/Cells Kit
The EZ2 RNA/miRNA Tissue/Cells Kit makes it easy and safe to purify total RNA – including miRNA and other small RNA – from cultured cells and various human or animal tissues. The process requires no use of phenols, and once the lysis step is completed, all remaining steps are automated with any EZ2 Connect instrument.