QIAmini DNA Blood Kit

The QIAmini DNA Blood (48) Kit contains reagents and single-use prefilled cartridges for the purification of genomic DNA from whole blood using QIAmini. This simple protocol consists of manual lysis followed by hands-free, magnetic-bead-based purification. Buffer AL and Proteinase K digestion enable complete lysis of blood samples. Following lysis, you transfer the lysate into the prefilled cartridge, loads the cartridge into the QIAmini and start the protocol. Prefilled cartridges minimize pipetting and reduce the risk of human error, leading to reproducible DNA recovery. Purified DNA is suitable for a wide range of downstream applications including qPCR, dPCR and DNA sequencing. The QIAmini DNA Blood Kit is designed for laboratories looking for easy-to-use, walk-away and standardized DNA purifications for research.

The QIAmini DNA Blood Kit together with QIAmini purifies high-yield DNA from whole blood samples (treated with EDTA, ACD, or heparin). The DNA yields are comparable to or higher than platforms and kits from alternative suppliers while having a lower overall total processing time. qPCR results also show similar Cqs for β-actin regardless of kit or automation instrument, indicating similar DNA purification quality for downstream analyses  Comparable yield and qPCR amplification using DNA from blood samples.

The DNA yield and quality is comparable whether purified by QIAmini DNA Blood Kit and or a trusted manual spin-column kit  Comparable DNA recovery from different donors.

Finally, QIAmini DNA Blood Kit greatly reduces the hands-on time spent performing purifications compared to manual extraction method and processing times for DNA extractions on QIAmini are lower or similar to those of alternative suppliers  Processing time comparison to the QIAmini DNA Blood Kit. 

The QIAmini DNA Blood (48) Kit uses magnetic-bead technology for automated purification of genomic DNA from whole blood. Enzymatic digestion and complete lysis easily achieved through mixing the blood samples with Proteinase K and Buffer AL. The lysate is then loaded into the prefilled cartridge where it is combined with the magnetic beads and isopropanol. The cartridge is prepared and put (with frame) in QIAmini. After the protocol is selected, QIAmini automatically performs nucleic acid binding to magnetic beads, wash steps, and elution.

The recommended elution volume is 100 µL, however adjustable are typically in a range of 50-200 µL. This cartridge-based workflow simplifies reagent handling and reduces manual pipetting during nucleic acid purification. The purified DNA can be either used immediately in downstream applications or stored for future use.

The simple and intuitive protocol requires only a few preparation and loading steps before fully automated purification begins  QIAmini DNA Blood Kit workflow:

1. Prepare samples homogenization and lysis with Proteinase K followed by buffer AL. Vortexing or efficient pipetting the mix ensures a consistent lysis.
2. Transfer the lysate into the prefilled cartridge in well 1 and add RNase-free water to well 5.
3. Load the cartridge onto the QIAmini workdeck and start the protocol.*
4. QIAmini performs binding, washing, and elution of genomic DNA.
5. Recover purified DNA from the elution well to a clean tube.

DNA purified using the QIAmini DNA blood Kit can be used in downstream applications including:

• Quantitative PCR (qPCR)
• Digital PCR (dPCR)
• NGS
• long-read sequencing