Cat. No. / ID: 69554
The QIAwave DNA Blood & Tissue Kit is an eco-friendlier version of our standard DNeasy Blood & Tissue Kit. The kit uses up to 62% less plastic and up to 58% less cardboard than our standard kit and waste tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. You can download the protocols from Resources or scan the QR code on the kit lid. While the packaging and components of our QIAwave Kit may look different, it’s as easy to use as our standard kit, and the chemistry and performance are identical. Please note that you will need sterile glass bottles to reconstitute the buffers.
In partnership with My Green Lab, we've assessed the environmental impact of this kit. My Green Lab ACT labels evaluate and score products on several sustainability criteria:
• responsible chemical management
• sustainable content within products and packaging materials
• disposal of the packaging at the end of life
Products are scored from 1 to 10 except for energy and water consumption, which are scored 1 point per kWh or gallon, respectively. A low score means a lower impact (see figures "QIAwave DNA Blood & Tissue Kit ACT label US, EU and UK").
The QIAwave Kit uses silica-based spin-columns for purification, and most samples can be lysed directly with proteinase K. This means:
• No mechanical disruption
• No organic extraction
• No alcohol precipitation
The standard protocol allows you to extract total DNA from animal blood and tissue. We’ve also developed protocols for other sample types to ensure you get reproducible quantities of high-quality DNA, whether you’re working in life science, genotyping or veterinary pathogen research. You can also automate your sample extraction on the QIAcube Connect.
The performance between the QIAwave DNA Blood & Tissue Kit and the DNeasy Blood & Tissue Kit is identical because the chemistry is the same. We’ve also shown that both kits outperform competitors’ kits (see figure “ QIAwave DNA Blood & Tissue Kit performance”).
The standard protocol of the QIAwave DNA Blood & Tissue Kit gives high yields of total DNA from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using QIAwave DNA Blood & Tissue " and figure "DNA yields"). However, we also offer optimized protocols to ensure high yields from nonstandard samples, such as:
Typical yields from animal tissues using QIAwave DNA Blood & Tissue Kit
|Mammalian blood||100 µL||3–6|
|Bird blood||5 µL||9–40|
|HeLa cells||2 x 106||15–25|
|Mouse tail||1.2 cm (tip)||10–25|
|Rat tail||0.6 cm (tip)||20–40|
|Pig ear||25 mg||10–30|
|Fish fin||20 mg||10–20|
|Fish spawn (mackerel)||10 mg||5–10|
We have also compared DNA yields obtained with the QIAwave DNA Blood & Tissue Kit (50) buffers, prepared by pipetting or pouring, and the DNeasy Blood & Tissue Kit (50) standard buffers. Both methods result in comparable DNA yields, as shown in the figure “ Handling buffer concentrates."
The QIAwave DNA Blood & Tissue Kit rapidly purifies total DNA (e.g., genomic, mitochondrial, and pathogen) from different sample types, including fresh or frozen animal tissues and cells, blood or bacteria.
The membrane combines the binding properties of a silica-based membrane with simple microspin technology. The DNA adsorbs to the membrane in the presence of high concentrations of chaotropic salt, which removes water from hydrated molecules in solution. The buffer conditions allow specific adsorption of DNA to the silica membrane and removal of contaminants and enzyme inhibitors.
The entire purification process does not require phenol or chloroform extraction or alcohol precipitation and involves minimal handling, which means you can easily process multiple samples simultaneously.
The QIAwave DNA Blood & Tissue Kit purifies DNA using DNeasy Mini Spin-Columns containing a silica-membrane. This makes the procedure fast and reproducible while eliminating manual and organic extraction or alcohol precipitatoin (see flowchart " QIAwave DNA Blood & Tissue Kit procedure").
Four simple steps to purify high-quality DNA:
QIAwave buffers come as concentrates that you can easily reconstitute by adding water and/or ethanol; please check the handbook for details. QIAwave DNeasy Mini Spin Columns and Waste Tubes come in individual bags and need to be preassembled before you start the protocol. This takes a little extra time, but it does reduce plastic waste.
The QIAwave DNA Blood & Tissue Kit can be automated on the QIAcube Connect using the DNeasy Blood & Tissue Kit protocols.
QIAwave DNA Blood & Tissue provides high-quality DNA, ready to use in all downstream assays, including applications in:
DNA was purified from 100 µL whole blood using DNeasy Mini Spin Columns. Goat: DNA was purified from 50 µL goat whole blood. Using more than 50 µL goat blood gave no significant increase in DNA yield. Chicken: DNA was purified from 5 µL chicken whole blood. Bird blood contains nucleated erythrocytes, giving higher DNA yields than mammalian blood.
|Applications||NGS, PCR, real-time PCR, genotyping|
|Elution volume||100–200 μL|
|Time per run or per prep||20 minutes|
|Main sample type||Blood, tissue|
|Sample amount||100 µL blood / 25 mg tissue / 5 x 106 cultured cells|
|Processing||Manual or QIAcube Connect|
|Yield||5 - 30 µg|
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein||DNA|
Yes, please follow the Supplementary Protocol 'Purification of total DNA from crude lysates using the DNeasy Blood & Tissue Kit' (DY15).
Yes, we have the following protocols:
It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.
Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA as anticoagulant to prevent the release of genomic DNA into the plasma fraction.
Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.
Yes, please follow the Supplementary Protocol 'Purification of total DNA from yeast using the DNeasy Blood & Tissue Kit' (DY13).
Yes, please follow either of the User-Developed Protocols:
Yes, please follow the Supplementary Protocol 'Purification of total DNA from insects using the DNeasy Blood & Tissue Kit' (DY14).
Alcohol precipitation is commonly used for concentrating, desalting, and recovering nucleic acids. Since less alcohol is required for isopropanol precipitation, this is the preferred method for precipitation of DNA from large volumes. In addition, isopropanol precipitation can be performed at room temperature, which minimizes co-precipitation of salt that interferes with downstream applications.
Tip: Use a buffer with a pH of 7.5–8.0, as DNA does not dissolve easily in acidic buffers. Often distilled water can have an acidic pH. The addition of EDTA protects the DNA from DNase digestion.
Tip: High-molecular-weight DNA, such as genomic DNA, should be redissolved very gently to avoid shearing. If the DNA pellet does not dissolve easily, heat at 55°C for 1–2 h with gentle shaking.
QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.
Yes, we have the following protocols:
The composition of Buffer AE is:
Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.