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Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
Yes, please follow the User-Developed Protocol 'Isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit; spin procedure' (DY07).
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DNeasy Blood & Tissue Kits
DNeasy Blood & Tissue Kits quickly extract DNA using a silica-based, phenol- and chloroform-free process in spin-column and 96-well-plate formats. For most samples, direct lysis with proteinase K eliminates the need for mechanical disruption, reducing hands-on time. Tailored protocols for specific samples ensure consistent extraction of high-quality DNA, perfect for life science, genotyping, and veterinary pathogen research. Kits can be automated on QIAcube Connect.
For a more sustainable alternative, the QIAwave DNA Blood & Tissue Kit, reduces plastic and cardboard usage by up to 62% and 58%, respectively. The kit includes waste tubes made from 100% recycled plastic, which can be reused throughout the procedure. QIAwave buffers are concentrated, decreasing plastic use in each bottle by up to 90%. Despite visual differences, the QIAwave Kit remains as user-friendly as the standard kit, with identical chemistry and performance. Note: Sterile glass bottles are required for buffer reconstitution.
In partnership with My Green Lab, we've assessed the environmental impact of the DNeasy Blood & Tissue Kit (50/250) and the QIAwave DNA Blood & Tissue Kit (50/250). My Green Lab ACT environmental impact factor labels aim to assess and rate products based on various sustainability criteria, including:
Manufacturing
Responsible chemical management
Sustainable content within products and packaging materials
Disposal of the packaging at the end of life
Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact – see figures “DNeasy Blood & Tissue Kit and QIAwave DNA Blood & Tissue Kit ACT environmental impact factor labels“ US ( 50/ 250), EU ( 50/ 250) and UK ( 50/ 250)."