FAQ-519
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
Yes, bisulfite containing methylation reactions can be cleaned up with our silica-based cleanup products, such as QIAquick and QIAEX II. Please see Goyon et al. (1994), 'Perpetuation of cytosine methylation in Ascobolus immersus implies a novel type of maintenance methylase', published in J Mol Biol. 1994 Jul 1;240(1):42-51, for a reference.
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QIAquick PCR Purification Kit
The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
QIAquick PCR Purification standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAquick PCR Purification protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Download more information.
QIAquick Nucleotide Removal Kit
The QIAquick Nucleotide Removal Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of oligonucleotides and DNA. Unincorporated nucleotides, salts, and other contaminants are removed and oligonucleotides (>17 nt) and DNA fragments ranging from 40 bp to 10 kb are purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–200 µl. The procedure is fully automated on the QIAcube Connect.
QIAquick Gel Extraction Kit
The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 µl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The QIAquick PCR & Gel Cleanup Kit also provides buffers for purification of PCR products >100 bp and DNA of up to 10 kb. The procedure can be fully automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
QIAEX II System
The QIAEX II system provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts. QIAEX II Suspension is added to solutions or solubilized agarose gel slices and binds DNA. The particles are collected by a brief centrifugation, washed and DNA of 40 bp to 50 kb is eluted in Tris buffer or water.