FAQ-3959
Is this available without glycerol?
Yes. Please contact your business development manager for a custom formulation.
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EnzScript Reverse Transcriptase
EnzScript Reverse Transcriptase is a Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase with point mutations in the RNase H domain that eliminate detectable RNase H activity. EnzScript can be used to generate first-strand cDNA from poly(A) mRNA or total RNA for use in downstream applications such as RT-PCR, cDNA cloning or library construction for RNA-Seq. The point mutations in the RNase H domain increase the thermostability of the enzyme and support greater cDNA yield of full-length transcripts than wild type M-MLV reverse transcriptase.
One unit of EnzScript Reverse Transcriptase is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo(dT) as a substrate.
EnzScript Reverse Transcriptase is supplied in 20 mM Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, <0.01% NP-40 stabilizer and 50% glycerol; pH 7.5 at 25°C.
EnzScript Reverse Transcriptase is supplied with 5X M-MLV Reverse Transcriptase RNase H– Reaction Buffer (cat. no. B7601) and 100mM DTT (cat. no. B9060).

