FAQ-3917
How long can reaction components incubate with VeraSeq 2.0 DNA Polymerase at room temperature prior to PCR cycling?
Successful amplification with high specificity and yield has been achieved after incubating all components for 24 hours prior to PCR cycling. For maximum success, use VeraSeq GC Buffer.
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VeraSeq™ 2.0 High-Fidelity DNA Polymerase
VeraSeq™ 2.0 High-Fidelity DNA Polymerase is an engineered, ultra-thermostable polymerase that delivers industry-leading speed, fidelity and robustness to PCR amplification. The novel enzyme can extend a kilobase of sequence in 15 seconds and with an accuracy 50 times higher than Taq DNA Polymerase.
Supplied in: 20 mM Tris·HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, Stabilizer and 50% glycerol; pH 7.4 at 25°C.
Supplied with: 5x VeraSeq™ Buffer II (B7102L) and 5x VeraSeq™ GC Buffer (B7130L).
You can also ask us about low glycerol or hot-start formulations.