FAQ-3884
Is it possible to scale up sample volume and reaction volume?
Although this is theoretically possible, the protocol is optimized for 20 µl reaction volume and all steps, volumes and reagents concentrations have been optimized to deliver optimal results in these conditions.
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QIAprep& Viral RNA UM Kit
The QIAprep& Viral RNA UM Kit is an innovative liquid-based approach to viral RNA epidemiology. The kit is optimized for ultrafast sample preparation and sensitive real-time PCR detection of enveloped RNA viruses such as coronaviruses from human samples (nasal, oro- and nasopharyngeal swabs) collected in non-fixation transport media like UTM, VTM, PBS, 0.9% NaCl, Virocult and eSwab. The kit is also compatible with neat saliva and gargle samples (please refer to the dedicated protocols below). Fewer and simpler workflow steps accelerate the turnaround time and increase testing frequency while decreasing plastic usage, cost and hands-on time. The method can be seamlessly integrated into automated workflows with standard lab equipment at all throughput needs. The QIAprep& Viral RNA QIAsymphony Kit can be used for automated sample preparation and assay set up on the QIAsymphony SP/AS. The QIAprep& Viral RNA QIAsymphony Kit requires the installation of a custom protocol available from QIAGEN Services.
The QIAprep& Viral RNA UM Kit can be used in conjunction with the SARS-CoV-2 N1+N2 Assay Kit, with the new SARS-CoV-2 Neo Kit or with the Multitarget Detection Assay for Respiratory Viruses: SARS-CoV-2, Flu A, Flu B, RSV A/B.
Want to try the QIAprep& Viral RNA UM Kit for the first time? Register to start your trial.
For labs that need to track currently relevant mutations of the virus, wet-lab tested LNA-based qPCR genotyping assays have been designed to detect the variants of concern (VOCs). The assay performance in terms of specificity and sensitivity has been verified using the QIAprep& Viral RNA UM Kit. View the assays here.