FAQ-385
What are the dissociation constants for the Anti-His Antibodies?
Anti·His Antibody epitopes and dissociation constants
| Antibody | Epitope | Dissociation constant Kd (M)* |
| RGS·His Antibody | RGSHHHH | 1 x 10–8 – 5 x 10–8 |
| Penta·His Antibody | HHHHH | 5 x 10–8 – 1 x 10–9 |
| Tetra·His Antibody | HHHH | 1 x 10–8 – 5 x 10–8 |
* Dissociation constants were measured using surface plasmon resonance (BIACORE) technology. The exact value of Kd is dependent on the individual 6xHis-tagged protein.
Related products
Anti·His Antibodies, BSA-free
Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. Each of the three variants detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma and contaminating immunoglobulins.