FAQ-362
Do pQE vectors contain the CAT gene?
The chloramphenicol acetyl transferase gene (CAT) present between t0 and T1 has no promoter and is not normally expressed. Depending on the bacterial strain and insert,low CAT activities may be detectable
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N-Terminus pQE Vector Set
This set provides 5 vectors (pQE-9, pQE-30, pQE-31, pQE-32, and pQE-40) for expression of N-terminally His-tagged proteins. pQE-30, pQE-31, and pQE-32 provide the multiple cloning site (MCS) in all three reading frames while pQE-9 has an alternative, shorter multiple cloning site. pQE-40 is designed for expression of DHFR-fusion proteins and is recommended for expression of poorly expressed proteins or short peptides, which are often prone to proteolysis, as DHFR enhances both stability and antigenicity. Since DHFR itself displays little immunogenicity in mouse and rat, DHFR-fusion proteins are ideal for epitope screening.
C-Terminus pQE Vector Set
This set provides 3 vectors (pQE-16, pQE-60, and pQE-70) for expression of C-terminally 6xHis-tagged proteins and is recommended for open reading frames with “pause sites”, which can cause premature termination. pQE-60 and pQE-70 allow the original start codon of the coding fragment to replace the ATG in the pQE vector, preserving the authentic N-terminus of the protein. These two constructs are created by introducing an NcoI and a SphI restriction site, respectively, at the ATG codon of the insert by PCR or mutagenesis. pQE-16 allows expression of C-terminally 6xHis-tagged DHFR-fusion proteins. DHFR (dihydrofolate reductase) enhances antigenicity and stability, and is recommended for poorly expressed proteins or short peptides prone to proteolysis. Since DHFR itself displays little immunogenicity in mouse and rat, DHFR-fusion proteins are ideal for epitope screening.