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Can proteins be extracted from PAXgene Tissue fixed specimens?
Yes. Supplementary protocols are available for the purification of full-length proteins from PAXgene Tissue fixed (PF) tissue and paraffin blocks using the Qproteome FFPE Tissue Kit (QIAGEN, cat. no. 37623). For more information, see the corresponding supplementary protocols under the Product Resources tab.
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PAXgene Tissue STABILIZER Concentrate
PAXgene Tissue STABILIZER is used for transport or storage of tissue specimens fixed with the PAXgene Tissue FIX Container (50 mL). After fixation of tissue samples with the PAXgene Tissue FIX Container (50 mL), PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins and morphology of the sample are now stable up to 7 days at room temperature, or for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. PAXgene Tissue STABILIZER Concentrate is only to be used in conjunction with tissue specimens previously fixed in PAXgene Tissue FIX.
PAXgene Tissue FIX Container (50 ml)
The PAXgene Tissue FIX Container (50 ml) enables fixation and stabilization of tissue specimens (up to 4 samples with a maximum size of 4 x 15 x 15 mm placed into 4 standard tissue cassettes, or a single tissue sample with a maximum size of 20 x 20 x 20 mm). PAXgene Tissue FIX Containers (50 ml) are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. PAXgene Tissue FIX rapidly penetrates and fixes the tissue, preserving tissue morphology. Fixation is comparable to formalin fixation, but without the destructive nucleic acid crosslinking and degradation. After fixation, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins, and morphology of the sample are stable up to 7 days at room temperature, and for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. PAXgene Tissue FIX Container (50 ml) is only to be used in conjunction with PAXgene Tissue STABILIZER.