Yes. The TAGzyme enzymes do not experience any loss in function after several freeze and thaw cycles. However, storage at -80°C is not necessary as storage at -20°C is adequate.
The TAGZyme Enzyme DAPase includes sufficient enzyme for highly specific and efficient His-tag removal from up to 10 mg His-tagged protein. The TAGZyme system can be used for His-tag removal from proteins containing an intrinsic DAPase stop point expressed using the TAGZyme pQE-2 vector.