3192 - Is QIAGEN Protease compatible with Buffer ATL?

Protease is a broad-specificity serine protease with high activity, cleaving preferentially at neutral and acidic residues. The enzyme is isolated from a recombinant Bacillus strain and offers broad substrate specificity in buffers commonly used in most DNA and RNA extraction procedures.   The protease is an economical alternative endopeptidase for isolation of native DNA and RNA from a variety of biological samples. QIAGEN Protease is dedicated for use in various QIAGEN kits (see list in the Principle section below).  For large-scale and sensitive applications, e.g., next-generation sequencing or diagnostics, we recommend high-purity Proteinase K as the preferred enzyme for proteolysis. 

DNeasy Blood & Tissue Kits quickly extract DNA using a silica-based, phenol- and chloroform-free process in spin-column and 96-well-plate formats. For most samples, direct lysis with proteinase K eliminates the need for mechanical disruption, reducing hands-on time. Tailored protocols for specific samples ensure consistent extraction of high-quality DNA, perfect for life science, genotyping, and veterinary pathogen research. Kits can be automated on QIAcube Connect. For a more sustainable alternative, the QIAwave DNA Blood & Tissue Kit, reduces plastic and cardboard usage by up to 62% and 58%, respectively. The kit includes waste tubes made from 100% recycled plastic, which can be reused throughout the procedure. QIAwave buffers are concentrated, decreasing plastic use in each bottle by up to 90%. Despite visual differences, the QIAwave Kit remains as user-friendly as the standard kit, with identical chemistry and performance. Note: Sterile glass bottles are required for buffer reconstitution. In partnership with My Green Lab, we've assessed the environmental impact of the DNeasy Blood & Tissue Kit (50/250) and the QIAwave DNA Blood & Tissue Kit (50/250). My Green Lab ACT environmental impact factor labels aim to assess and rate products based on various sustainability criteria, including: Manufacturing Responsible chemical management Sustainable content within products and packaging materials Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact – see figures “DNeasy Blood & Tissue Kit and QIAwave DNA Blood & Tissue Kit ACT environmental impact factor labels“  US ( 50/ 250),  EU ( 50/ 250) and  UK ( 50/ 250)."