FAQ-315
How can QIAGEN Protease and Proteinase K be inactivated?
QIAGEN Protease is inactivated by incubation at 70°C for 15 minutes.
To our knowledge, Proteinase K cannot be completely heat-inactivated. Even when incubating at 95°C for 10 minutes, some enzymatic activity remains. This will not negatively affect the QIAamp Procedure, since the enzyme will be efficiently removed by the wash steps in the protocols.
Related products
Proteinase K
Proteinase K is a broad-spectrum, subtilisin-related serine protease derived from the fungus Parengyodontium album (Tritirachium album). The enzyme is active across a wide range of temperatures, pH values and denaturing conditions (including SDS and EDTA). Its high specific activity enables efficient digestion of proteins, including DNases and RNases, during nucleic acid preparation without compromising DNA or RNA integrity.
The recombinant 28.9 kDa enzyme is expressed in Komagataella phaffii (Pichia pastoris) and extensively purified for high solubility, specific activity and low residual DNA content. QIAGEN Proteinase K is available in ready-to-use solution or lyophilized powder formats, including an Ultrapure option.
Proteinase K - Solution can be provided in bulk and as a customized product with an extended shelf life of up to 3 years. We also offer Proteinase K - Powder as a lyophilized powder in a range of pack sizes to maximize long-term stability, maintain high enzymatic activity and permit ambient temperature shipping.
Contact us for more information about custom and OEM options.
QIAGEN Protease is an economical endopeptidase alternative to Proteinase K and is suitable for isolation of native DNA and RNA from a variety of biological samples, e.g., blood and cultured cells.
QIAGEN Protease
Protease is a broad-specificity serine protease with high activity, cleaving preferentially at neutral and acidic residues. The enzyme is isolated from a recombinant Bacillus strain and offers broad substrate specificity in buffers commonly used in most DNA and RNA extraction procedures.
The protease is an economical alternative endopeptidase for isolation of native DNA and RNA from a variety of biological samples. QIAGEN Protease is dedicated for use in various QIAGEN kits (see list in the Principle section below).
For large-scale and sensitive applications, e.g., next-generation sequencing or diagnostics, we recommend high-purity Proteinase K as the preferred enzyme for proteolysis.
QIAamp DNA Kits
QIAamp DNA Kits provide silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids or washed cells from urine. In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. Mechanical homogenization is not required as the tissues are lysed enzymatically. DNA purification from 1–12 samples can be automated on the QIAcube Connect using the dedicated QIAamp DNA Mini QIAcube Kit. Purification of DNA using the QIAamp DNA Micro Kit and QIAamp DNA Mini Kit is also automatable on the QIAcube Connect.
QIAamp DNA Mini standard protocols can also be executed using the TRACKMAN Connected system.
QIAamp DNA Blood Kits
QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 μl up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 1–12 samples on the QIAcube Connect.