Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?

The FastLane Cell cDNA Kit provides a fast and simple procedure for preparing first-strand cDNA directly from cultured cells without RNA purification. The kit is supplied with wash and lysis buffers for preparing lysates and stabilizing RNA, gDNA Wipeout Buffer for eliminating genomic DNA contamination, and all reaction components for fast and efficient cDNA synthesis. The FastLane Cell cDNA Kit is ideal for experiments requiring rapid, high-throughput gene expression analysis.

FastLane Cell RT-PCR Kits accelerate and streamline real-time RT-PCR and are ideal for experiments requiring rapid high-throughput gene expression analysis. The kits provide a set of buffers for preparing FastLane lysates from cultured cells. In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA. FastLane lysates are used directly in real-time one-step RT-PCR and multiplex, real-time one-step RT-PCR. A variety of detection formats are available, including singleplex detection with SYBR Green or sequence-specific probes or multiplex detection with sequence-specific probes (with or without ROX passive reference dye).