FAQ-2839
Which end of the PCR primer for pyrosequencing should be biotinylated?
In pyrosequencing, the 5' end should be biotinylated, regardless of whether the forward or reverse primer is biotinylated.
Related products
Pyrosequencing: PyroMark Q48 Autoprep
Users of PyroMark Q96 ID, the PyroMark Q24 and PyroMark Q24 Advanced and PyroMark Q24 MDx instruments should be aware that reagents for these instruments will be discontinued after August 2025, December 2026 and February 2027, respectively. Assays should be migrated to the PyroMark Q48 Autoprep instrument to improve the Pyrosequencing workflow with automation and advanced methylation, mutation and SNP quantification.
PyroMark Q96 Systems
The PyroMark Q96 MD has been discontinued and is no longer supported.
The PyroMark Q96 ID was discontinued in 2016 and the instrument is no longer actively sold. Existing instruments will be supported until the end of August 2025. Reagents will continue to be available until the end of August 2025.
To future-proof your Pyrosequencing assays, consider upgrading your work to using a PyroMark Q48 Autoprep System, which is actively sold and supported by QIAGEN.