FAQ-2800
What is the composition of Buffer RDD?
The exact composition of Buffer RDD is proprietary. Buffer RDD is an important component of the RNase-Free DNase Set, which is used in combination with most RNeasy Kits. The composition and salt concentration of Buffer RDD provides efficient on-column digestion of DNA and also ensures that the RNA remains bound to the column.
Related products
RNeasy Kits
RNeasy Kits are the gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured cell samples using silica membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect.
RNeasy Plant Mini Kit
The RNeasy Plant Mini Kit includes QIAshredder spin columns for homogenizing and filtering viscous plant or fungal lysates, and RNeasy spin columns for purifying up to 100 μg of high-quality RNA using silica-membrane technology. Purification can be fully automated on the QIAcube Connect. The kit can also be used in combination with a TissueRuptor or TissueLyser system, which provide efficient disruption and homogenization of plant samples.
RNeasy MinElute Cleanup Kit
The RNeasy MinElute Cleanup Kit enables cleanup and concentration of RNA from enzymatic reactions or other samples using specialized RNeasy MinElute spin columns based on silica-membrane technology. The kit can also be used to desalt RNA samples. Up to 45 µg RNA can be purified in a volume as low as 10 µl. Purification can be fully automated on the QIAcube Connect.
RNase-Free DNase Set
The QIAGEN RNase-Free DNase Set is guaranteed RNase-free, quality-controlled, and optimized for use with RNeasy procedures and with QIAamp RNA Blood Mini procedures. Generally, DNase digestion is not required for RNA purified with RNeasy Kits since the silica-gel–membrane, spin-column technology efficiently removes the majority of the DNA without DNase treatment. However, more complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA. Buffer RDD, included in the set, is optimized for on-column DNase digestion of 15 minutes at 20–30°C. The buffer is also well-suited for efficient DNase digestion in solution. The RNase-Free DNase Set provides efficient on-column digestion of DNA during RNA purification from cells and tissues using RNeasy Kits and the QIAamp RNA Blood Mini Kit. The DNase is efficiently removed in subsequent wash steps. For DNase treatment when using the RNeasy 96 Kit, please contact QIAGEN Technical Services or your local distributor for a separate, optimized protocol. The QIAGEN RNase-Free DNase Set is delivered as a stable, lyophilized enzyme. The RNase-Free DNase Set provides 1500 Kunitz units.