FAQ-2577
Which human target is used for the quantification in the Investigator Quantiplex Kits?
For all Investigator Quantiplex Kits, the 4NS1C target is used, a multi-copy target which was validated in an external study. It is represented by about 20 copies per haploid genome and is located on different autosomal chromosomes.
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Investigator Quantiplex Kit
Investigator Quantiplex Kits are ready-to-use quantitative real-time PCR assays for human identity testing and forensic applications with conventional 2 µL or flexible 1-18 µL sample input (Investigator Quantiplex Pro FLX Kit). Developed for superior sensitivity and reaction speed, they are designed to establish whether a sample contains sufficient and intact human DNA to enable STR or SNP analysis by Capillary Electrophoresis or NGS. An internal amplification control (IC) is used to test successful amplification and identify the presence of PCR inhibitors. The Investigator Quantiplex Pro technology further enables quality assessment to provide reliable information on the degradation status of your sample.
Investigator Quantiplex Kits meet ISO 18385 requirements.
Investigator Quantiplex Pro FLX Kit
The Investigator Quantiplex Pro FLX Kit is the next generation of quantification in human identification testing. The kit combines trusted multicopy markers for advanced assessment of quantity, degradation and inhibition, with novel lyophilized chemistry in 96-well breakable optical plates (see figure Investigator Quantiplex Pro FLX features: Ease-of-use). It enables for more flexibility in template input volumes starting from 1 µL to up to 18 µL per reaction, and thus offers increased sensitivity by higher input volumes. Purified DNA or sample lysates processed with the Investigator Casework GO! Kit can be used for qPCR. The high template input volumes and fast reaction setup times make the new Investigator Quantiplex Pro FLX Kit ideal for high throughput application and screening approaches where highest sensitivity is pivotal. The kit is validated on Applied Biosystems 7500 or QuantStudio 5 Real-Time PCR Systems.
Investigator Quantiplex Pro FLX meet ISO 18385 requirements.
Want to try the Investigator Quantiplex Pro FLX Kit for the first time? Request a quote for a trial kit.