FAQ-205
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
DNA fragments purified with the QIAGEN DNA Cleanup Systems, i.e., the QIAquick PCR Purification Kit, the MinElute Reaction Cleanup Kit, the QIAEX II Gel Extraction Kit etc. may float out of the loading wells of agarose gels due to residual ethanol carried over from the wash step with Buffer PE (despite the addtition of glycerol-containing loading buffer).
Use either of the following options to remove residual ethanol from the eluate:
- re-purify the sample using a QIAquick-, or MinElute column, or QIAEX II resin
- incubate the eluate at 56°C for 10 min to evaporate the ethanol
- dry down the sample in a vacuum centrifuge, and resuspend the pellet in a small volume of sterile water
Related products
QIAquick PCR Purification Kit
The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
QIAquick PCR Purification standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAquick PCR Purification protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Download more information.
QIAquick Nucleotide Removal Kit
The QIAquick Nucleotide Removal Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of oligonucleotides and DNA. Unincorporated nucleotides, salts, and other contaminants are removed and oligonucleotides (>17 nt) and DNA fragments ranging from 40 bp to 10 kb are purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–200 µl. The procedure is fully automated on the QIAcube Connect.
QIAquick Gel Extraction Kit
The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 µl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The QIAquick PCR & Gel Cleanup Kit also provides buffers for purification of PCR products >100 bp and DNA of up to 10 kb. The procedure can be fully automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
QIAquick 96 PCR Purification Kits
QIAquick 96 PCR Purification Kits provide 96-well plates, buffers, and collection tubes for high-throughput silica-membrane-based purification of PCR products >100 bp in size. DNA up to 10 kb is purified using a simple and fast bind–wash–elute procedure and an elution volume of 60–80 µl (resulting in an eluate volume of 40–60 µl). The cleanup procedure can be fully automated on the BioRobot Universal workstation using the QIAquick 96 PCR BioRobot Kit.
QIAEX II System
The QIAEX II system provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts. QIAEX II Suspension is added to solutions or solubilized agarose gel slices and binds DNA. The particles are collected by a brief centrifugation, washed and DNA of 40 bp to 50 kb is eluted in Tris buffer or water.
MinElute Reaction Cleanup Kit
The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions. The spin columns are designed to allow elution in very small volumes (as little as 10 µl), delivering highly concentrated DNA in high yields. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
MinElute PCR Purification Kit
The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp – 4 kb in size. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.
MinElute Gel Extraction Kit
The MinElute Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments of 70 bp – 4 kb from up to 400 mg gel slices. The spin columns are designed to allow elution in very small volumes (as little as 10 µL), delivering high yields of highly concentrated DNA. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute Gel Extraction Kit can be automated on the QIAcube Connect.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.