FAQ-1937
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
The QIAsymphony RNA Kit enables purification of RNA from cultured cells and easy-to-lyse tissues, such as kidney, liver, and spleen on the QIAsymphony SP. A large-volume protocol is available that enables processing of larger sample amounts.
- Cultured cells: up to 3x106 cells (standard protocol); up to 1x107 (large volume protocol)
- Tissues: up to 20 mg of most tissues (standard protocol); up to 50 mg (large-volume protocol)
For particularly DNA-rich tissues, such as spleen or thymus, we recommend either using no more than half the maximum amount, or using DNase at a higher concentration (see the QIAsymphony RNA Handbook; additional DNase required).
Related products
QIAsymphony RNA Kit
The QIAsymphony RNA Kit enables automated purification of total RNA, or total RNA including miRNA and other small RNAs, from 1–96 samples on the QIAsymphony SP. Total RNA can be purified from animal cells, tissues, or FFPE tissues, using very small amounts of starting material (≥100 cells) up to 1 x 107 cells or 50 mg of tissue. Optimized protocols provide high yields of pure total RNA, or total RNA including miRNA, and flexible elution volumes allow RNA concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
QIAsymphony SP/AS instruments
The QIAsymphony SP is a versatile nucleic acid purification instrument providing high-quality nucleic acids compatible with with most common downstream assay technologies, such as real-time PCR, digital PCR, and next generation sequencing (NGS). The large range of a dedicated QIAsymphony kits enables optimized purification of genomic DNA, cell-free circulating DNA, RNA, bacterial and viral nucleic acids from a wide range of starting materials. The QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, which, in combination with the Rotor-Gene Q and QIAGEN real-time and end-point PCR kits, enables you to optimize your PCR workflow and maximize your efficiency.
Interested in benchtop instruments for automated extraction of DNA or RNA instead? Compare all of our lab automation instruments in our Instrument Selection Table.