FAQ-1550
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
Yes, we have the following protocols:
- Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
- Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
- Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).
Related products
TissueLyser Adapter Sets
TissueLyser Adapter sets are designed for efficient and user-friendly disruption of samples in 1.2 mL Collection Microtubes (racked), 2 mL tubes, 5 mL tubes, 50 mL tubes or 96-well plates on the TissueLyser III & II.
The TissueLyser Adapter Set 2 x 24 contains a pair of TissueLyser Reaction-Tube Holders, 24-Tube. Each holder holds up to 24 microcentrifuge tubes (2 mL), totaling up to 48 samples per run. The use of Safe-Lock tubes is strongly recommended (e.g., Eppendorf 2 mL Safe-Lock micro test tubes).
The TissueLyser Adapter Set 2 x 96 is for use with QIAGEN Collection Microtubes (racked) and Collection Microtube Caps, totaling up to 192 samples per run. Collection Microtubes are 1.2 mL in volume.
The 5 mL Tube Adapter Set is for use with 5 mL bead tubes (e.g., PowerWater DNA Bead Tubes). Each set holds up to 16 tubes.
The 50 mL Tube Adapter Set is for use with 50 mL tubes (e.g., 50 mL Falcon tubes). Each adapter can hold up to 4 tubes.
The Plate Adapter Set is for use with two 96-well plates; it is compatible with the PowerBead Pro Plates.
The 2 mL Tube Holder Set is for use with 2 mL tubes. It can only be used with the Plate Adapter Set. Each holder can fit up to 48 tubes.
RNeasy Lipid Tissue Mini Kit
The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using the QIAcube Connect. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.
QIAzol Lysis Reagent
QIAzol Lysis Reagent is optimized for lysis of fatty tissues. The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation.