FAQ-154
What is the recommended culture medium for the QIAprep System?
Luria-Bertani (LB) broth is the recommended culture medium for use with QIAprep Kits, since richer broths such as TB (Terrific Broth) or 2x YT lead to extremely high cell densities, which can overload the purification system. Please review the section 'Culture Media' of Appendix A in the QIAprep Miniprep Handbook or visit our Plasmid Resource Center for additional information on optimal plasmid culturing and extraction conditions for all QIAGEN Plasmid Purification Kits.
Related products
QIAprep Spin Miniprep Kit
The QIAprep Spin Miniprep Kit is designed for isolating high-purity plasmid or cosmid DNA, offering up to 20 µg yields for various molecular biology applications like sequencing and cloning. Higher yields (up to 30 µg) can be achieved using the High-Yield Supplementary Protocol. The kit is automatable on the QIAcube Connect.
Want to try the QIAprep Spin Miniprep Kit for the first time? Request a quote for a trial kit.
For optimal results, we recommend pairing this kit with the QIAvac 24 Plus system.
QIAprep 96 Turbo Kits
The QIAprep 96 Turbo Kits provide QIAprep 96 and TurboFilter 96 plates for purification of up to 20 µg molecular biology grade plasmid DNA per well. For optimal results it is recommended to use this product together with QIAvac 96.
Plasmid Buffers
Plasmid Buffers are used in plasmid DNA purification procedures. They include Buffer P1 (resuspension buffer), Buffer P2 (lysis buffer), Buffer N3 and Buffer P3 (neutralization buffers), Buffer QC (wash buffer) Buffer QBT (equilibration buffer) and Buffer QF (elution buffer).