FAQ-153896
Can the 2nd Prot K step be omitted?
The 2nd Proteinase K step improves lysis efficiency and yields, in particular for difficult-to-lyse tissue material. Hence, omission of this step may result in decreased yields.
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Increase your recovery of high-quality DNA from FFPE tissue with the QIAamp DNA FFPE Advanced Kits’ xylene-free, no-wash deparaffinization, double-lyse protocol, and UCP (ultra-clean production) spin column technology. Remove C→U transitions of nucleic acids with the kits’ optional UNG digest to optimize your recovered DNA for NGS analysis. You can also automate the QIAamp DNA FFPE Advanced protocols on the QIAcube Connect.>