FAQ-141
How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?
Assuming that the ligation conditions are correct, QIAEX II particle carryover may affect ligation efficiency. Under low salt conditions, enzymes may bind to QIAEX II particles, thus reducing enzymatic activity in the ligation reaction. To remove the particles, centrifuge the tube containing the DNA for 30 seconds before pipetting the DNA.
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QIAEX II System
The QIAEX II system provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts. QIAEX II Suspension is added to solutions or solubilized agarose gel slices and binds DNA. The particles are collected by a brief centrifugation, washed and DNA of 40 bp to 50 kb is eluted in Tris buffer or water.