Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?

RNeasy Plus Kits are next generation QIAGEN RNA extraction kits, combining high-quality RNA purification with effective gDNA removal with unique gDNA Eliminator columns or plates. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. The RNeasy Plus Micro Kit is specially designed for limited amounts of samples and isolates up to 45 µg pure total RNA The RNeasy Plus Mini Kit purifies up to 100 µg total RNA (>200 nt) from a single extraction with efficient gDNA removal. Both kits can be automated on the QIAcube Connect and samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system (available separately). The RNeasy Plus 96 Kit is for high-throughput purification of total RNA from cultured cells grown in 96-well plates. 

The RNeasy MinElute Cleanup Kit enables cleanup and concentration of RNA from enzymatic reactions or other samples using specialized RNeasy MinElute spin columns based on silica-membrane technology. The kit can also be used to desalt RNA samples. Up to 45 µg RNA can be purified in a volume as low as 10 µl. Purification can be fully automated on the QIAcube Connect.