Do you have a protocol for purification of total DNA from crude lysates?

DNeasy Blood & Tissue Kits quickly extract DNA using a silica-based, phenol- and chloroform-free process in spin-column and 96-well-plate formats. For most samples, direct lysis with proteinase K eliminates the need for mechanical disruption, reducing hands-on time. Tailored protocols for specific samples ensure consistent extraction of high-quality DNA, perfect for life science, genotyping, and veterinary pathogen research. Kits can be automated on QIAcube Connect. For a more sustainable alternative, the QIAwave DNA Blood & Tissue Kit, reduces plastic and cardboard usage by up to 62% and 58%, respectively. The kit includes waste tubes made from 100% recycled plastic, which can be reused throughout the procedure. QIAwave buffers are concentrated, decreasing plastic use in each bottle by up to 90%. Despite visual differences, the QIAwave Kit remains as user-friendly as the standard kit, with identical chemistry and performance. Note: Sterile glass bottles are required for buffer reconstitution. In partnership with My Green Lab, we've assessed the environmental impact of the DNeasy Blood & Tissue Kit (50/250) and the QIAwave DNA Blood & Tissue Kit (50/250). My Green Lab ACT environmental impact factor labels aim to assess and rate products based on various sustainability criteria, including: Manufacturing Responsible chemical management Sustainable content within products and packaging materials Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact – see figures “DNeasy Blood & Tissue Kit and QIAwave DNA Blood & Tissue Kit ACT environmental impact factor labels“  US ( 50/ 250),  EU ( 50/ 250) and  UK ( 50/ 250)."   

The QIAwave DNA Blood & Tissue Kit is an eco-friendlier version of our standard DNeasy Blood & Tissue Kit. The kit uses up to 62% less plastic and up to 58% less cardboard than our standard kit and waste tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. You can download the protocols from Resources or scan the QR code on the kit lid. While the packaging and components of our QIAwave Kit may look different, it’s as easy to use as our standard kit, and the chemistry and performance are identical. Please note that you will need sterile glass bottles to reconstitute the buffers. In partnership with My Green Lab, we've assessed the environmental impact of this kit. My Green Lab ACT labels evaluate and score products on several sustainability criteria: • Manufacturing• Responsible chemical management• Sustainable content within products and packaging materials• Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored 1 point per kWh or gallon, respectively. A low score means a lower impact (see figures "QIAwave DNA Blood & Tissue Kit ACT label  US 50/250,  EU 50/250 and  UK 50/250"). The QIAwave Kit uses silica-based spin-columns for purification, and most samples can be lysed directly with proteinase K. This means: • No mechanical disruption• No organic extraction• No alcohol precipitation The standard protocol allows you to extract total DNA from animal blood and tissue. We’ve also developed protocols for other sample types to ensure you get reproducible quantities of high-quality DNA, whether you’re working in life science, genotyping or veterinary pathogen research. You can also automate your sample extraction on the QIAcube Connect.