FAQ-1251
Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?
Yes, please follow the protocol "Transfection of Macrophage Cell Lines, Including J774.A1 and RAW 264.7, with siRNA in 24-Well Plates" in the HiPerFect Transfection Reagent Handbook.
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HiPerFect Transfection Reagent
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations. In addition to siRNA, HiPerFect Transfection Reagent is ideally suited to transfection of miRNA mimics or inhibitors. Cell-type-specific protocols using HiPerFect Transfection Reagent are available at the TransFect Protocol Database.