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Where can I find a protocol for cleanup of already purified plasmid DNA?
When using the silica-based QIAprep Spin Miniprep Kit, a protocol is contained in the QIAprep Miniprep Handbook, in Appendix C: Special Applications. The protocol is called: 'Purification of plasmid DNA prepared by other methods'.
For our anion-exchange based Plasmid Purification Kits, a protocol can be accessed online at our Plasmid Resource Center, and is called 'Re-Purification of Plasmid DNA Prepared by Methods other than QIAGEN Tips'.
Related products
QIAprep Spin Miniprep Kit
The QIAprep Spin Miniprep Kit is designed for isolating high-purity plasmid or cosmid DNA, offering up to 20 µg yields for various molecular biology applications like sequencing and cloning. Higher yields (up to 30 µg) can be achieved using the High-Yield Supplementary Protocol. The kit is automatable on the QIAcube Connect.
Want to try the QIAprep Spin Miniprep Kit for the first time? Request a quote for a trial kit.
For optimal results, we recommend pairing this kit with the QIAvac 24 Plus system.
QIAGEN Plasmid Kits
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. See more information regarding principle below.
QIAfilter Plasmid Kits
QIAfilter Plasmid Kits provide anion-exchange-based plasmid purification with bacterial lysate clearing by filtration. Purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.
The QIAfilter Plasmid Mega Kit (cat. no. 12281) and the QIAfilter Plasmid Giga Kit (cat. no. 12291) will be discontinued on June 30, 2021. They remain available as long as stocks last.The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. Visit the QIAGEN plasmid kits web page to learn more.>
HiSpeed Plasmid Kits
HiSpeed Plasmid Kits provide fast, large-scale, anion-exchange-based plasmid DNA preparation without centrifugation. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.
Plasmid Buffers
Plasmid Buffers are used in plasmid DNA purification procedures. They include Buffer P1 (resuspension buffer), Buffer P2 (lysis buffer), Buffer N3 and Buffer P3 (neutralization buffers), Buffer QC (wash buffer) Buffer QBT (equilibration buffer) and Buffer QF (elution buffer).