FAQ-1020
Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
Yes, please follow the protocol in the RNAprotect Bacteria Reagent Handbook in Appendix D: Stabilization and Purification of Bacterial RNA Using RNAprotect Bacteria Reagent, the RNeasy Lipid Tissue Mini Kit, and the TissueLyser.
Related products
RNeasy Lipid Tissue Mini Kit
The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using the QIAcube Connect. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.
RNAprotect Reagents and Tubes
RNAprotect Reagents and Tubes provide immediate RNase inactivation and stabilization of RNA in tissues, cultures of Gram-positive and Gram-negative bacteria and sorted or cultured cells to preserve the gene expression profile and enable reproducible purification of high-quality RNA. Samples are stabilized at room temperature prior to RNA isolation procedures.
QIAzol Lysis Reagent
QIAzol Lysis Reagent is optimized for lysis of fatty tissues. The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation.