QIAseq Targeted DNA Sequencing Panels
Digital DNA sequencing to confidently detect low-frequency variants
QIAseq Targeted DNA Panel (12)
Cat no. / ID. 333502
Kit containing ALL reagents (except indexes) for targeted DNA sequencing; fixed panel for 12 samples; less than 100 genes
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Panel
QIAseq Targeted DNA Panel (standard)
QIAseq Targeted DNA Pro Panel
QIAseq Targeted cfDNA Ultra Panel
Panel type
Fixed
Custom
Size
12
96
| Popular Products | GeneGlobe ID |
|---|---|
| QIAseq Targeted DNA Human Actionable Solid Tumor Panel | DHS-101Z |
| QIAseq Targeted DNA Human Breast Cancer Panel | DHS-001Z |
| QIAseq Targeted DNA Human BRCA1 and BRCA2 Panel | DHS-102Z |
| QIAseq Targeted DNA Human HRR Panel | DHS-110Z |
| QIAseq Targeted DNA Human Pharmacogenomics Panel | DHS-104Z |
Features
- Confident low-frequency variant detection with molecular barcodes and UMIs
- Efficient target enrichment, including challenging GC-rich regions
- Flexible options for tissue, FFPE and cfDNA samples
- Standard, Pro and cfDNA Ultra workflows for different research needs
- Catalog and custom panel designs
Product Details
QIAseq Targeted DNA Sequencing Panels offer three options built on a shared digital DNA sequencing foundation.
| QIAseq Targeted DNA Panel | QIAseq Targeted DNA Pro Panel | QIAseq Targeted cfDNA Ultra Panel | |
|---|---|---|---|
| Best for | A proven, versatile starting point for targeted DNA sequencing across diverse sample types | Faster, automation-friendly targeted DNA sequencing with expanded variant analysis | Ultra-sensitive targeted sequencing for detecting extremely rare variants in cell-free DNA (cfDNA) from blood for liquid biopsy research |
| Ideal sample | DNA from tissue, blood, FFPE or cfDNA | DNA from tissue, blood, FFPE and cfDNA | Cell-free DNA (cfDNA) |
| Sensitivity | Reliable detection of low-frequency variants (SNVs, small indels, CNVs, select structural variants with characterized breakpoints, LOH), >90% sensitivity on detecting small variants down to 0.5% | Reliable detection of low-frequency variants (SNVs, small indels, CNVs, select structural variants with characterized breakpoints, LOH), >90% sensitivity on detecting small variants down to 0.5% | Ultra-low-frequency somatic variant detection, down to 0.1% VAF, with >99% specificity |
| Input needed | 10–80 ng (up to 250 ng FFPE) | 10–80 ng (up to 250 ng FFPE) | As little as 5–80 ng cfDNA |
| Time to library | ~ 9 hours | ~ 6 hours | ~8 hours |
| Hands-on time | ~3 hours | ~1.5 hours | ~2 hours |
| Sequencing platforms | Illumina, Ion Torrent, Element Biosciences | Illumina, Ion Torrent, Element Biosciences | Illumina, Element Biosciences |
| Catalog numbers | Fixed (12, 96): 333502, 333505 Custom (96): 333525 | Fixed (12, 96): 333651, 333655 Custom (96): 333675 | Fixed (12, 96): 334051, 334055 Custom (96): 334405, 334455 |
*Sizes indicate the number of reactions per kit.
Performance
QIAseq Targeted DNA Sequencing Panels combine molecular barcoding with highly specific target enrichment for sensitive, reliable detection of low-frequency variants.
QIAseq Targeted DNA Panels:
Accuracy and sensitivity. Molecular barcodes help reduce PCR and sequencing errors. In QIAGEN testing, panels achieved >90% sensitivity for NA12878 SNPs and indels at 1% variant frequency, with<15 false positives per megabase under specified tiled-primer conditions.
Specificity and uniformity. Optimized enrichment delivers highly specific, uniform coverage, including challenging GC-rich regions.
Flexibility. Supports SNVs, small indels and CNVs, with catalog and custom designs for exons, hotspots, SNPs, intronic regions and promoters.
Workflow. The workflow from extracted DNA to sequencing-ready libraries can be completed in 9 hours.
QIAseq Targeted DNA Pro Panels:
Sensitive variant detection. Integrated UMIs support confident detection of known and novel variants.
Challenging samples. Built-in FFPE repair and optimized chemistry support fragmented DNA and GC-rich targets.
Faster workflow. Sequencing-ready libraries in <6 hours with minimal hands-on time and automation-friendly enzymatic cleanup.
Expanded variant analysis. Supports SNVs, small indels, CNVs, select structural variants and lossof heterozygosity (LOH).
QIAseq Targeted cfDNA Ultra Panels:
Ultra-sensitive detection. Detects variants down to 0.1% VAF using integrated UMIs, high-fidelity chemistry and error correction.
High specificity and uniformity. Delivers >99% specificity with QIAGEN CLC Genomics Workbench, with >99% coverage uniformity and >90% target specificity in supporting data.
Low-input, high-multiplex performance. Uses 5–80 ng human cfDNA and supports up to 20,000 primers per panel, including GC-rich targets.
Efficient workflow. Sequencing-ready libraries within 8 hours with <2 hours of hands-on time.
Principle
PCR duplicates and errors introduced during amplification and sequencing can make it difficult to confidently detect variants present at low frequencies in the starting DNA.
All QIAseq Targeted DNA Sequencing workflows leverage UMI-enabled digital DNA sequencing and Single Primer Extension (SPE) technology to deliver highly specific target enrichment and confident variant detection.
Procedure
QIAseq Targeted DNA Sequencing Panels follow a streamlined workflow from extracted DNA to variant analysis, with specific steps and sequencing options depending on the panel family.
- Start with your DNA. Use DNA from supported sample types, including tissue, FFPE, plasma/serum or cell-free DNA (cfDNA), depending on the panel.
- Add molecular barcodes. Unique molecular indices (UMIs) tag original DNA molecules before target amplification, helping distinguish true variants from PCR and sequencing errors.
- Enrich your targets. QIAseq single-primer enrichment selectively amplifies the genes and regions included in your panel.
- Index and sequence. Add sample indices for multiplexing, then sequence on a compatible Illumina or Ion Torrent platform, depending on the panel family.
- Analyze and interpret. Use QIAseq analysis solutions or QIAGEN CLC Genomics Workbench for barcode-aware variant calling, with QCI Interpret available for further variant annotation and interpretation.
Applications
QIAseq Targeted DNA Sequencing Panels can be used to analyze a variety of DNA variants across a range of sample types and research applications. Supported capabilities vary by panel family.
DNA variants
- SNVs
- Small indels
- CNVs
- Select structural variants with well-characterized breakpoints
- Loss of heterozygosity (LOH)
Sample types
- FFPE
- Plasma/serum
- Fresh or frozen tissue
- Cell lines
- Human cell-free DNA (cfDNA)*
Applications
- Profiling DNA variants in solid and hematologic malignancies
- Hotspot detection in solid tumors
- Examination of variants in mitochondrial DNA
- Pain and ADME pharmacogenomics
- Human identity and paternity testing
- Assessment of germline variants for inherited diseases
- Profiling of exonic regions in BRCA1 and BRCA2
- Liquid biopsy and early cancer profiling*
- Minimal residual disease (MRD) research*
- Research into treatment resistance*
*QIAseq Targeted cfDNA Ultra Panels are designed specifically for human cfDNA.
Supporting data and figures
Workflow
Isolated DNA, as low as 20 ng, is enzymatically fragmented to generate small pieces of dsDNA. This is followed by the library construction step, in which IL-N7 adapters, molecular barcodes, and sample indexes are incorporated into DNA fragments generated in the previous step. Library fragments now serve as templates for target enrichment using QIAseq Enrichment Technology. In this step, targets are enriched using a single gene-specific primer and a universal forward primer. The final step is library amplification and sample indexing (for dual indexing) using the IL-S5 sample index primer and a universal primer.
Resources
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks & Protocols (5)
Additional Resources (1)
Brochures & Guides (1)
Validation & Technical Documentation (1)
Scientific documents (4)
Software-related downloads (8)
Publications
Non-invasive detection of actionable mutations in advanced non-small-cell lung cancer using targeted sequencing of circulating tumor DNA.
Lung Cancer; 2018; 124:154-159 2018 Aug 8 PMID:30268455
smCounter2: an accurate low-frequency variant caller for targeted sequencing data with unique molecular identifiers.
Bioinformatics; 2019; 35 (8):1299-1309 2019 Apr 15 PMID:30192920
Correlation of genomic alterations between tumor tissue and circulating tumor DNA by next-generation sequencing.
J Cancer Res Clin Oncol; 2018; 144 (11):2167-2175 2018 Sep 10 PMID:30203147
Detecting very low allele fraction variants using targeted DNA sequencing and a novel molecular barcode-aware variant caller.
BMC Genomics; 2017; 18 (1):5 2017 Jan 3 PMID:28049435
Dysplastic Lipoma: A Distinctive Atypical Lipomatous Neoplasm With Anisocytosis, Focal Nuclear Atypia, p53 Overexpression, and a Lack of MDM2 Gene Amplification by FISH; A Report of 66 Cases Demonstrating Occasional Multifocality and a Rare Association With Retinoblastoma.
Am J Surg Pathol; 2018; 42 (11):1530-1540 2018 Nov PMID:30001242