Multi-Analyte ELISArray Kits

For the simultaneous detection of up to 12 cytokines or chemokines in multiple biological samples using an ELISA

Features

  • Detection of up to 12 cytokines or chemokines
  • High sensitivity and linearity
  • Screened and verified capture and detection antibodies
  • Uses standard ELISA equipment

Product Details

This product will be discontinued as of November 12, 2020.

Multi-Analyte ELISArray Kits are the simplest simultaneous multi-analyte ELISA available. Highly specific capture antibodies for key cytokines and chemokines are arrayed in a 96-well plate. One easy ELISA protocol allows the detection of 12 cytokines or chemokines simultaneously in multiple samples.

Performance

One example of a Multi-Analyte ELISArray Kit application is as follows. A Multi-Analyte ELISArray Kit was used to profile multiple cytokines secreted by stimulated human peripheral blood mononuclear cells (PBMCs). The time-dependent (0, 6, 18, 24, and 48 hours) patterns of 8 Th1/Th2 cytokine inductions by human PBMCs were montored in response to PMA (50 µg/ml) and ionomycin (1 µg/ml) (see figure " Patterns of Th1/Th2 cytokine induction". The relative amount of each of the 8 cytokines was also profiled using the Multi-Analyte ELISArray Kit.

Large amounts of IL-2 and IFNγ, and to a lesser extent TNFα, are induced throughout, and apparently beyond, the time course tested. Only more moderate amounts of IL4 and IL5 are induced, reaching a maximum at approximately 24 hours of stimulation. The even lower levels of IL10 and IL13 that are secreted also reach a maximum at 24 hours and appear to decrease again at later times. The amount of IL12 is not detectable after 48 hours of constant stimulation.

See figures

Principle

Multi-Analyte ELISArray Kits profile 12 cytokines in 6 cell supernatant (media), serum, or plasma samples using one simple protocol and one standardized development time (see figure " Multi-Analyte ELISArray plate layout"). Multi-Analyte ELISArray Kits provide researchers with a rapid assessment of the level of multiple proteins (cytokines and chemokines) in multiple samples.

Commercially available capture and detection antibodies are screened to achieve the highest possible sensitivity and linearity for each cytokine or chemokine assay. All assays are optimized under uniform conditions to allow simultaneous detection without compromising performance (see figure " Optimized ELISA conditions enable the same development or incubation time").

See figures

Procedure

Multi-Analyte ELISArray Kits use a standard sandwich ELISA protocol (see flowchart " ELISArray protocol"). There is no requirement for special equipment; kits are compatible with a standard ELISA plate reader.

See figures

Applications

Multi-Analyte ELISArray Kits are highly suited for research into autoimmune and immune disorders, cancer, immunology, infectious diseases, and many other areas of biological and medical research. They can be used in any application typically analyzed using individual ELISA kits.

Supporting data and figures

Resources

Safety Data Sheets (1)

FAQ

Do the ELISArray Kits work on species other than human, mouse or rat?
QIAGEN has not validated the ELISArray kits on any species not specifically described in the product literature
FAQ ID -2906
Can I use lysates or tissue homogenates with the ELISArray kits?
No. QIAGEN does not support using either tissue homogenates or cell lysates because often these samples include detergents that inhibit the analyte-antibody interaction.
FAQ ID -2905
What is the Limit of Detection (LOD) for an ELISArray Kit?
The LOD for each analyte in each kit is specified on the product specification sheet in units of picograms per milliliter.
FAQ ID -2907
How much sample is required for each well for an ELISArray Kit?
50 µl is required. If you do not know the approximate concentration of the analyte of interest, QIAGEN recommends measuring an undiluted sample and a 100-fold diluted sample. There is a high probability that one of these two sample concentrations will be within the linear range of the assay.
FAQ ID -2908
What is the concentration of SureENTRY Transduction Reagent?
The concentration of SureENTRY Transduction Reagent is 10 mg/ml.
FAQ ID - 3708
What is the linear range of the Multi-Analyte ELISArray Kits?
OD450 readings between 0 and 2.5 are within the linear range for all of the analytes. The positive control will not always fall within the linear range, but the samples must fall within this range for accurate relative quantization (i.e. fold changes between samples).
FAQ ID -2909