EZ1&2 RNA Tissue Mini Kit

For automated purification of high-quality total RNA

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EZ1&2 RNA Tissue Mini Kit (48)

Cat. No. / ID: 959034

For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water

$674.00

Quantity

The name of this kit was changed from "EZ1 RNA Tissue Mini Kit" to "EZ1&2 RNA Tissue Mini Kit" to demonstrate that it is compatible with the new EZ2 Connect instruments as well as the EZ1 Advanced XL. No changes were made to the well-established kit technology.

The EZ1&2 RNA Tissue Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Purification of up to 30 µg total RNA
Reliable processing of up to 10 mg easy-to-lyse tissues
RNase-free setup for reliable results
Scalable elution volumes
Easy protocol setup

Product Details

The EZ1&2 RNA Tissue Mini Kit contains all required reagents and labware for automated purification of total RNA from up to 10 mg standard tissue samples using magnetic particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.

The EZ1&2 RNA Tissue Mini Kit is automated with the EZ2 Connect (1–24 samples per run). This kit can also be used with the EZ1 Advanced XL (1–14 samples per run).

Performance

The yields and high quality of total RNA purified using the EZ1&2 RNA Tissue Mini Kit are consistent and reproducible (see figures " Efficient purification and reproducible yields" and " Consistent highly pure RNA").

See figures

Efficient purification and reproducible yields.

Consistent highly pure RNA.

Principle

Automated purification with the EZ1&2 RNA Tissue Mini Kit requires use of one of the following:

the EZ2 Connect
the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card

EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1&2 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.

Procedure

Tissues are first lysed and homogenized in Buffer RLT. RNA is then purified in one step on the instrument following the automated EZ1&2 RNA procedure. For certain RNA applications that are sensitive to very small amounts of DNA, an integrated DNase digestion step removes genomic DNA contamination. Total RNA is eluted in 200 µl, 100 µl, or 50 µl, ready to use in all downstream applications.

Applications

The high-quality RNA obtained using the EZ1&2 RNA Tissue Mini Kit is suited for use in many applications, such as:

Gene expression analysis using real-time RT-PCR technologies
Gene expression analysis using microarray technologies
Oncology and pathology research

Supporting data and figures

Efficient purification and reproducible yields.

Using the EZ1&2 RNA Tissue Mini procedure, total RNA was purified from 10 mg samples of rat kidney and liver and from 5 mg samples of rat pancreas, spleen, and lung. RNA was purified from 3 replicate samples of each tissue type. Yields of total RNA were determined by absorbance (A₂₆₀) with correction for background (A₃₂₀). CV kidney = 15%; CV liver = 1%; CV pancreas = 8%; CV spleen = 10%; CV lung = 22%.

Specifications

Features	Specifications
Applications	PCR, real-time RT-PCR, microarray
Time per run or per prep	40 minutes
Technology	Magnetic particles
Elution volume	50–200 µl
Processing	Automated
Format	Sample tube
Stabilization	Yes
For automated processing	EZ2 Connect instruments or EZ1 Advanced XL
Main sample type	Tissue samples
Sample amount	5–10 µg
Yield	<30 µg
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA

Resources

Fully automated low- to medium-throughput purification of nucleic acids

For purification of total RNA from tissue and cells using EZ1 instruments

FAQ

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

No, the new EZ1 RNA Kits can only be used with updated protocols programmed on the new EZ1 RNA Card V2.0.

The new card contains all protocols available for the isolation of RNA on the BioRobot EZ1 with the new kits:

Purification of total RNA from cells using the EZ1 RNA Cell Mini Kit, with and without DNase digest.
Purification of total RNA from standard tissue using the EZ1 RNA Tissue Mini Kit, with and without DNase digest.
Purification of total RNA from any type of tissue using the EZ1 RNA Universal Tissue Kit, with and without DNase digest.

Likewise, the former EZ1 RNA Cell Mini Kit (cat. no. 958134), EZ1 RNA Tissue Mini Kit (cat. no. 959134) and EZ1 RNA Universal Tissue Kit (cat. no. 956136) are not compatible with the new EZ1 RNA Card (cat. no. 9015590).

FAQ ID -1025

Yes, please follow the Supplementary Protocol 'Isolation of total nucleic acids from animal and human tissues using the EZ1 RNA Tissue Mini Kit'.

FAQ ID -975

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

FAQ ID -528

Yes, please follow the User-Developed Protocol 'Purification of total RNA from paraffin-embedded sections using the EZ1 RNA Tissue Mini Kit' (MA33).

FAQ ID -976

Yes, please follow the Supplementary Protocol 'Purification of DNA from fungi using the EZ1 DNA Tissue Kit' (MA35).

FAQ ID -992

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246