QIAamp 96 PowerFecal QIAcube HT Kit

For automated high-throughput purification of genomic DNA from fresh or frozen stool samples that are high in PCR inhibitors

Features

  • Simple and reliable automated processing for cost and time savings
  • No hazardous organic chemicals required
  • Efficient removal of PCR inhibitors
  • High sensitivity in downstream assays

Products

The QIAamp 96 PowerFecal QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAcube HT Plasticware

Cat. No. / ID: 950067

Plasticware for 480 typical preps on QIAcube HT: 5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
$331.00
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S-Blocks (24)

Cat. No. / ID: 19585

96-well blocks with 2.2 ml wells, 24 per case
$358.00
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Pathogen Lysis Tubes L

Cat. No. / ID: 19092

50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX
$270.00
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Product Details

The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using the QIAcube HT. The novel combination of MOBIO's patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.

The QIAamp 96 PowerFecal QIAcube HT Kit is to be discontinued shortly. Try the DNeasy 96 PowerSoil Pro QIAcube HT Kit – for improved performance. Higher yields, better purity, more accurate representation of the microbial diversity.

Performance

QIAcube HT and dedicated QIAcube HT purification kits let users increase sample purification throughput without having to compromise quality or reliability.

Effective removal of inhibitors
Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples.

Principle

The QIAamp 96 PowerFecal QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.

Procedure

The QIAamp 96 PowerFecal QIAcube HT procedure is fast and simple with the QIAcube HT instrument. PCR inhibitors are removed by the MOBIO proprietary Inhibitor Removal Technology (IRT) with two precipitation steps. A combination of mechanical disruption and proteinase K digestion ensure complete lysis of even difficult-to-lyse organisms, resulting in high concentrations of unbiased DNA.

Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE. No hazardous organic chemicals are used, increasing laboratory and personal safety.

Applications

The high-quality nucleic acids are ready to be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease and metabolic research applications.

Specifications

FeaturesSpecifications
Time per run96 samples in approximately 120 minutes 24 samples in approximately 60 minutes
Sample amountUp to 200 mg fresh or frozen stool
Throughput24-96 samples in increments of 8 samples
Elution volume100 μl

Resources

Operating Software (1)
Files necessary for configuring the QIAcube HT Prep Manager Software to process QIAamp 96 PowerFecal QIAcube HT Kits. Not compatible with QIAcube HT Operating Software 4.17.

For kit configuration installation instructions, please refer to the QIAcube HT User Manual  chapter 4.4.5.
Kit Handbooks (1)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Quick-Start Protocols (1)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
Brochures & Guides (2)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699