Environmental or human samples are broken down and homogenized. Cells are lysed by both mechanical and chemical means and the DNA is captured on a 96-well silica membrane in a spin plate format, washed and eluted. The DNA is then ready for downstream applications such as qPCR or next-generation sequencing.
Sequencing results reveal higher alpha diversity as measured by observed operational taxonomic units (OTUs) compared to other tested methods (See figure Significantly increased DNA yields and better quality compared to other suppliers while offering comparable yields to other QIAGEN products.).
DNA isolated using the DNeasy 96 PowerSoil Pro identified more OTUs for both bacteria (See figure Increased bacterial representation.) and fungi (See figure Increased fungal representation.) compared with the legacy DNeasy PowerSoil HTP 96.