EZ1 DSP Virus Kit

For automated purification of DNA from 1–24 or 1–14 human biological specimens using the EZ2 Connect MDx or the EZ1 Advanced XL

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EZ1 DSP Virus Kit (48)

Cat. No. / ID:  62724

For 48 viral nucleic acid preps: Prefilled Reagent Cartridges, Disposable Tip-Holders, Disposable Filter-Tips, Sample Tubes, Elution Tubes, buffers, carrier RNA
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$584.00
The EZ1 DSP Virus Kit is intended for in vitro diagnostic use.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • For in vitro diagnostic use
  • High analytical sensitivity even with low viral titers
  • Rapid and flexible purification of 1–24 or 1–14 samples per run
  • Standardized processing ensures reproducible results

Product Details

The EZ1 DSP Virus Kit contains all required reagents and labware for automated purification of viral nucleic acids and bacterial DNA from human serum, plasma, cerebrospinal fluid (CSF), stool and nasopharyngeal swabs in UTM using magnetic-particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the EZ2 Connect MDx or the EZ1 Advanced XL. The EZ1 DSP Virus Kit provides exact performance specifications, assuring highly reliable viral nucleic acid purification.

Performance

High analytical sensitivity with lower detection limits

The EZ1 DSP Virus Kit provides simultaneous purification of viral nucleic acids and bacterial DNA from human serum, plasma, CSF, stool and nasopharyngeal swabs in UTM. Efficient purification of viral RNA and DNA results in sensitive analytical detection in polymerase chain reaction (PCR), on a variety of real-time thermal cyclers.

Principle

Automated sample preparation enables efficient, standardized purification of nucleic acids. The EZ1 Advanced and the EZ1 Advanced XL enable purification of nucleic acids using proven magnetic-particle technology for use in routine in vitro diagnostic applications. Reagent production is subjected to stringent quality control assuring reliable and robust performance.
An easy-to-use instrument, prefilled and sealed reagent cartridges, and simple protocol selection and worktable setup help to prevent handling errors and allow the system to be operated by anyone — from the novice to the expert. Manual handling of potentially infectious samples is minimized, ensuring safety to the user and reliable processing of samples. Purified viral DNA and RNA are ready to use in sensitive downstream diagnostic assays based on enzymatic amplification, such as PCR and reverse transcriptase PCR (RT-PCR) for viral load monitoring.

Procedure

The EZ2 Connect MDx or the EZ1 Advanced XL in combination with the proven EZ1 DSP Virus Kit enables purification of viral nucleic acids and bacterial DNA from 1–24 (EZ2 Connect MDx) or 1–14 (EZ1 Advanced XL) samples of human serum, plasma, CSF, stool and nasopharyngeal swabs in UTM. All processing steps are performed by the instrument, from piercing the reagent cartridge to elution of highly pure nucleic acids. The innovative design of the instrument means that the pipet tips function as separation chambers, increasing the efficiency of magnetic purification and eliminating the need for centrifugation steps. The streamlined procedure provides robust walkaway purification of nucleic acids with minimal hands-on time.

Applications

With the EZ2 Connect MDx or the combination of the EZ1 Advanced XL DSP Virus Card and the EZ1 Advanced XL, the EZ1 DSP Virus Kit provides a fully automated procedure for simultaneous purification of viral nucleic acids and bacterial DNA from human serum, plasma, CSF, stool and nasopharyngeal swabs in UTM. Viral nucleic acids purified using the EZ1 DSP Virus Kit are ready to use in downstream diagnostic assays based on enzymatic amplification, such as PCR and RT-PCR.

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time RT-PCR, genotyping
Elution volume75–50 µl
Main sample typeSerum, plasma
CE/FDA/IVD compatibleCE/IVD
ProcessingAutomated
For automated processingEZ2 Connect MDx and EZ1 Advanced XL
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA and bacterial DNA
Sample amount100–400 µl
TechnologyMagnetic particles

Resources

Kit Handbooks (3)
For the Directive 98/79/EC (IVDD) compliant kit (kit version 4)
EZ1 DSP Virus Kit Handbook_V5_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices
New 2D barcodes on EZ1 DSP reagent cartridges
Safety Data Sheets (1)

FAQ

How long can eluates be stored after sample preparation using the EZ1 instruments or EZ2 Connect MDx and the EZ1 DSP Virus Kit?
For short-term storage of up to 24 hours, it is recommended to store the purified viral nucleic acids or bacterial DNA at 2–8°C. For long-term storage of more than 24 hours, it is recommended to store at –80°C for up to 12 months or –20°C for up to 12 weeks. Stability of nucleic acids might be different for the specific downstream application being used and needs to be self-validated by the user.
FAQ ID - 3897