FAQ-2057
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
SNP Genotyping using TaqMan® probes is a unique application requiring the development of a unique reaction chemistry. The Type-it Fast SNP Probe PCR Kit allows wide separation of genotype clusters and reliable amplification of any difficult genomic region for high call rates.
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Type-it Fast SNP Probe PCR Kit
The Type-it Fast SNP Probe PCR Kit is based on highly specific HotStarTaq Plus DNA Polymerase and a newly developed buffer system, both of which enable reliable and clear allelic discrimination. The combination of all components provided in the master mix result in improved accuracy and highly specific probe binding. The Type-it Fast SNP Probe PCR Kit is validated using TaqMan® SNP Genotyping Assays and enables reproducible SNP genotyping — even with difficult SNP loci (e.g., GC rich) or low amounts of starting template.