FAQ-2004
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
An ideal amplicon size for bisulfite converted DNA (e.g., using EpiTect Bisulfite Kits) is up to 250 bp. However, we amplified fragments up to 700 bp successfully using bisulfite converted DNA as a template for the EpiTect MSP Kit. Note that the time for the elongation step needs to be increased for such long amplicons.
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EpiTect Bisulfite Kits
EpiTect Bisulfite Kits enable complete conversion of unmethylated cytosines to uracils and subsequent purification in 6 hours. The EpiTect 96 Bisulfite procedure takes less than 7 hours. The highly sensitive method utilizes innovative protection against DNA degradation that guarantees sensitive results, even from 1 ng DNA, and ensures high conversion rates of over 99%. Due to its unique DNA Protect technology, EpiTect Bisulfite converted DNA is highly suitable for whole bisulfitome amplification using EpiTect Whole Bisulfitome Kits. This enables reliable amplification of DNA in cases where bisulfite converted DNA is limited. EpiTect Bisulfite Kits include a spin-column or 96-well format procedure, and can be processed using a centrifuge or vacuum manifold. In addition, the EpiTect Bisulfite Kit can be fully automated on the QIAcube Connect.