Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?

The QIAprep Spin Miniprep Kit is designed for isolating high-purity plasmid or cosmid DNA, offering up to 20 µg yields for various molecular biology applications like sequencing and cloning. Higher yields (up to 30 µg) can be achieved using the High-Yield Supplementary Protocol. The kit is automatable on the QIAcube Connect. Want to try the QIAprep Spin Miniprep Kit for the first time? Request a quote for a trial kit. For optimal results, we recommend pairing this kit with the QIAvac 24 Plus system.

Plasmid Buffers are used in plasmid DNA purification procedures. They include Buffer P1 (resuspension buffer), Buffer P2 (lysis buffer), Buffer N3 and Buffer P3 (neutralization buffers), Buffer QC (wash buffer) Buffer QBT (equilibration buffer) and Buffer QF (elution buffer).

The QIAwave Plasmid Miniprep Kit is an eco-friendlier version of our standard QIAprep Spin Miniprep Kit. The QIAwave Kit uses up to 22% less plastic and up to 14% less cardboard than our standard kit and offers waste tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers come as concentrates, reducing the amount of plastic by up to 93% per bottle. To save paper, there are no printed protocols in the kit. You can download the protocols from the resources list or scan the QR code inside the kit box. Although the kit packaging and components of our QIAwave Kit may look different, it’s as easy to use as our standard kit, and the chemistry and performance are identical. Please be aware that you will need sterile glass bottles to store the reconstituted buffers. In partnership with My Green Lab, we've also assessed the environmental impact of this kit. My Green Lab ACT labels are designed to evaluate and score products on several sustainability criteria. These include: • Manufacturing• Responsible chemical management• Sustainable content within products and packaging materials• Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact – see figures "QIAwave Plasmid Miniprep Kit ACT environmental impact factor label US 50/250, EU 50/250 and UK 50/250 "). The QIAwave Plasmid Miniprep Kit is designed to isolate up to 20 μg high-purity plasmid or cosmid DNA for routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. You can achieve even higher yields (up to 30 μg) using the QIAprep High-Yield Supplementary Protocol. We recommend using this kit with the QIAvac 24 Plus for optimal results.