MinElute PCR Purification Kit
For purification of up to 5 μg PCR products (70 bp to 4 kb) in low elution volumes
- Very small elution volumes
- Fast procedure and easy handling
- High, reproducible recoveries
- Gel loading dye for convenient sample analysis
The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp – 4 kb in size. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube.
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MinElute PCR Purification Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
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28004
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MinElute PCR Purification Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
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28006
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The MinElute PCR Purification Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
MinElute procedure.|Efficient primer removal.|GelPilot Loading Dye.|MinElute membrane.|pH Indicator Dye.|Spin column handling options — D.|Spin column handling options — E.|Spin column handling options — C.|Spin column handling options — B.|Spin column handling options — A.|
This simple bind–wash–elute procedure ensures greater convenience.|Analysis of PCR products before (b) and after (a) purification with the MinElute PCR Purification Kit. Samples were analyzed on a 1.2% TAE agarose gel. M: markers.|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
|Unique MinElute membrane assembly.
|pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
|QIAvac 24 plus.
|QIAcube.
|Manifold with luer connectors.
|QIAvac 24.
|Microcentrifuge.
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Performance
The MinElute PCR Purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples (see figure " Efficient primer removal").
The MinElute PCR Purification Kit provides spin columns for PCR product cleanup. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick PCR Purification Kit.)
Principle
The MinElute PCR Purification Kit contains a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries.
Gel loading dye
To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").
Procedure
The MinElute System uses a simple bind-wash-elute procedure. Binding buffer is added directly to the PCR sample or other enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Handling
MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters and can also be fully automated on the QIAcube (see figures "Spin column handling options A, B, C, D, and E").
Applications
DNA fragments purified with the MinElute System are ready for direct use in all applications, including:
- Sequencing, including next generation sequencing
- Microarray analysis
- Ligation and transformation
- Restriction digestion
- Labeling
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Feature
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Specifications
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Binding capacity
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5 µg
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Elution volume
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10 µl
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Format
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Tube
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Fragment size
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70 bp – 4 kb
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Processing
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Manual
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Recovery: oligonucleotides dsDNA
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Recovery: oligonucleotides, dsDNA
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Removal <10mers 17–40mers dye terminator proteins
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Removal <40mers
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Sample type: applications
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DNA, oligonucleotides: PCR reactions
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Technology
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Silica technology
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I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them? AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro
FAQ ID - 3560
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For MinElute PCR Purification Kit, MinElute Gel Extraction Kit, MinElute Reaction Cleanup Kit
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